Literature summary for 3.4.99.B1 extracted from

Casey, P.J.; Rando, R.R.
Isoprenylated protein peptidase Rce1 (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 2123-2127.

No PubMed abstract available

Search for more literature for 3.4.99.B1

Application

Application	Comment	Organism
medicine	excision of RCE1 from skin carcinoma cell line retards the growth of cells significantly, and this effect was exaggerated by co-treatment with farnesyl-transferase inhibitor	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	RCE1 deficient mice generated, loss of the enzyme results in late embryonic lethality	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
beta-(2-naphthyl)-Ala-CH2Cl	affinity labeling agent of the enzyme	Bos taurus
additional information	not inhibited by chyostatin, DTT, E-64, 1,10-phenanthroline and PMSF, Tos-Lys-CH2Cl	Bos taurus
N-Boc-S-farnesyl-L-cys-CH2Cl	-	Bos taurus
N-Boc-S-farnesyl-L-cysteine aldehyde	potent competetive inhibitor	Bos taurus
p-chloromercuribenzoate	inactivates the partially purified enzyme, with 0.005 mM substantial inhibition	Bos taurus
pseudo peptide R (CH2-NH)	-	Bos taurus
Tos-Phe-CH2Cl	affinity labeling agent of the enzyme	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0057	-	N-acetyl-S-farnesyl-L-cysteinyl-L-valyl-L-isoleucyl-L-serine	-	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-
microsome	-	Bos taurus	-	-

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Homo sapiens	-	-	-
Mus musculus	-	-	-
Saccharomyces cerevisiae	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
carcinoma cell line	from skin	Mus musculus	-
liver	-	Bos taurus	-
MEF cell	-	Mus musculus	-
additional information	Sf9 cell	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00000251	-	with N-acetyl-S-farnesyl-Cys+Val-Ile-Ser as substrate	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a-factor-CaaX + H2O	CaaX proteolysis of all three forms of Ras and other CaaX proteins is absent in RCE1 deficient mice, RCE1 is required for correct localization of these proteins	Mus musculus	a-factor-C + aaX	-	?
N-acetyl-S-farnesyl-Cys-Val-Ile-Ser + H2O	-	Bos taurus	N-acetyl-S-farnesyl-Cys + Val-Ile-Ser	-	?
N-Boc-S-farnesyl-L-Cys-Val-Ile-Met + H2O	-	Bos taurus	N-Boc-S-farnesyl-L-Cys + Val-Ile-Met	-	?

Synonyms

Synonyms	Comment	Organism
isoprenylated protein peptidase	-	Mus musculus
isoprenylated protein peptidase	-	Homo sapiens
isoprenylated protein peptidase	-	Saccharomyces cerevisiae
isoprenylated protein peptidase	-	Bos taurus
RCE1	-	Mus musculus
RCE1	-	Homo sapiens
RCE1	-	Saccharomyces cerevisiae
RCE1	-	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000085	-	pseudo peptide R (CH2-NH)	-	Bos taurus
0.002	-	N-Boc-S-farnesyl-L-cysteine aldehyde	-	Bos taurus
0.03	-	N-Boc-S-farnesyl-L-cys-CH2Cl	-	Bos taurus

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Release 2023.1 (January 2023)