Literature summary for 3.4.25.2 extracted from

Ramachandran, R.; Hartmann, C.; Song, H.K.; Huber, R.; Bochtler, M.
Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY) (2002), Proc. Natl. Acad. Sci. USA, 99, 7396-7401.

Search for more literature for 3.4.25.2

Activating Compound

Activating Compound	Comment	Organism	Structure
HslU	protease HslV is activated by the ATPase HslU. Mutations in hslV that disrupt the interaction with the C termini of HslU invariably lead to inactive enzyme	Haemophilus influenzae

Protein Variants

Protein Variants	Comment	Organism
DELTA83-92	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae
DELTA86-91	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is unchanged	Haemophilus influenzae
K28A	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae
R35A	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae
R86D	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae
R89A/K90A	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae
R89D	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is 40-60% of the activity of the wild-type enzyme	Haemophilus influenzae
R89D/K90E	hydrolysis of casein and SulA-MBP is less than 20% of the activity of the wild-type enzyme, hydrolysis of benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is higher than that of the wild-type enzyme	Haemophilus influenzae
V112A	hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme	Haemophilus influenzae

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O	-	Haemophilus influenzae	benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin	-	?
casein + H2O	-	Haemophilus influenzae	?	-	?

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Release 2023.1 (January 2023)