Application | Comment | Organism |
---|---|---|
analysis | simultaneous labeling and detection of constitutive 20S proteasome subunits using an affinity-based probe cocktail and application to biological samples | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bortezomib | - |
Homo sapiens | |
MG-132 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
acetyl-Arg-hSer-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
0.0159 | - |
acetyl-hArg-Pro-2-fluoro-Phe-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
0.0432 | - |
acetyl-hArg-Pro-Abu-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
0.051 | - |
acetyl-betaAla-Met(sulfone)-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
HEK-293F cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Arg-hSer-Thr-Arg-7-amido-4-methylcoumarin + H2O | substrate for trypsin-like activity | Homo sapiens | acetyl-Arg-hSer-Thr-Arg + 7-amino-4-methylcoumarin | - |
? | |
acetyl-betaAla-Met(sulfone)-Thr-Arg-7-amido-4-methylcoumarin + H2O | substrate for trypsin-like activity | Homo sapiens | acetyl-betaAla-Met(sulfone)-Thr-Arg + 7-amino-4-methylcoumarin | - |
? | |
acetyl-hArg-Pro-2-fluoro-Phe-Asp-7-amido-4-methylcoumarin + H2O | substrate for caspase-like activity | Homo sapiens | acetyl-hArg-Pro-2-fluoro-Phe-Asp + 7-amino-4-methylcoumarin | - |
? | |
acetyl-hArg-Pro-Abu-Asp-7-amido-4-methylcoumarin + H2O | substrate for caspase-like activity | Homo sapiens | acetyl-hArg-Pro-Abu-Asp + 7-amino-4-methylcoumarin | - |
? | |
acetyl-Phe-Thr(Bzl)-His(3-benzyloxymethyl)-Leu-7-amido-4-methylcoumarin + H2O | substrate for chymotrypsin-like activity | Homo sapiens | acetyl-Phe-Thr(Bzl)-His(3-benzyloxymethyl)-Leu + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.343 | - |
acetyl-hArg-Pro-Abu-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
1.844 | - |
acetyl-hArg-Pro-2-fluoro-Phe-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
2.233 | - |
acetyl-betaAla-Met(sulfone)-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
2.837 | - |
acetyl-Arg-hSer-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.61 | - |
acetyl-Phe-Thr(Bzl)-His(3-benzyloxymethyl)-Leu-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
31.11 | - |
acetyl-hArg-Pro-Abu-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
43.79 | - |
acetyl-betaAla-Met(sulfone)-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
116.1 | - |
acetyl-hArg-Pro-2-fluoro-Phe-Asp-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
301 | - |
acetyl-Arg-hSer-Thr-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens |