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Literature summary for 3.4.25.1 extracted from

  • Gruba, N.; Wysocka, M.; Brzezinska, M.; Debowski, D.; Rolka, K.; Martin, N.; Lesner, A.
    Novel internally quenched substrate of the trypsin-like subunit of 20S eukaryotic proteasome (2016), Anal. Biochem., 508, 38-45 .
    View publication on PubMed
Search for more literature for 3.4.25.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 ABZ-Val-Val-Ser-(4-guanidino-Phe)-Ala-Met-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens
0.0177
-
 ABZ-Val-Val-Ser-Arg-Ser-Leu-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ABZ-Val-Val-Ser-Arg-Ser-Leu-Gly-Tyr(3-NO2)-NH2 + H2O synthetic, internally quenched substrate Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
 ABZ-Val-Val-Ser-(4-guanidino-Phe)-Ala-Met-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens
16.5
-
 ABZ-Val-Val-Ser-Arg-Ser-Leu-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
934
-
 ABZ-Val-Val-Ser-Arg-Ser-Leu-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens
1980
-
 ABZ-Val-Val-Ser-(4-guanidino-Phe)-Ala-Met-Gly-Tyr(3-NO2)-NH2 20S proteasome, pH 8.2, 37°C Homo sapiens