Inhibitors | Comment | Organism | Structure |
---|---|---|---|
calpeptin | inhibits calpain-like activity | Mus musculus | |
clastolactacystin-beta-lactone | - |
Mus musculus | |
epoxomicin | inhibits trypsin-like activity | Mus musculus | |
NH4Cl | lysosomal inhibitor | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
eight-week-old female mice | - |
Mus musculus C57BL/6 | - |
eight-week-old female mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | differentiated from C6 glioma cells | Mus musculus | - |
brain | - |
Mus musculus | - |
cerebellum | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O | for trypsin-like activity | Mus musculus | ? | - |
? | |
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O | for trypsin-like activity | Mus musculus C57BL/6 | ? | - |
? | |
Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | for chymotrypsin-like activity | Mus musculus | ? | - |
? | |
Suc-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | for chymotrypsin-like activity | Mus musculus C57BL/6 | ? | - |
? | |
Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O | for caspase-like activity | Mus musculus | Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin | - |
? | |
Z-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O | for caspase-like activity | Mus musculus C57BL/6 | Z-Leu-Leu-Glu + 7-amino-4-methylcoumarin | - |
? | |
Z-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | for calpain-like activity | Mus musculus | Z-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
Z-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | for calpain-like activity | Mus musculus C57BL/6 | Z-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | reduced proteasomal activity contributes to the accumulation of carbonylated proteins in chronic experimental autoimmune encephalomyelitis. Chymotrysin-like and caspase-like activities of the 20S proteasome are impaired in chronic experimental autoimmune encephalomyelitis, while the amount of proteasome is unchanged, determination of proteasome failure by the build-up of ubiquitinated proteins, mostly within astrocytes, diminished activity of the 20S proteasome is a major contributor to the accumulation of carbonylated proteins in astrocytes of chronic experimental autoimmune encephalomyelitis mice | Mus musculus |