Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epoxomicin | - |
Rattus norvegicus | |
NMDA | exposure causes the disassembly of 26S proteasomes and dissociation of E3, i.e. KCMF1, HUWE1, and UBE3A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
synapse | - |
Rattus norvegicus | 45202 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate | ? | - |
? | |
additional information | Rattus norvegicus Sprague-Dawley | mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Sprague-Dawley | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native 26S proteasomes from cytosolic or synaptosomal extracts of brain cortex by nickel and glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | proteasome composition in brain, overview | Rattus norvegicus | - |
cerebellar cortex | - |
Rattus norvegicus | - |
additional information | a higher proportion of doubly-capped 26S proteasome (19S-20S-19S) in the brain cortex than in the liver or kidney | Rattus norvegicus | - |
neuron | hippocampal | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate | Rattus norvegicus | ? | - |
? | |
additional information | mass spectrometric analysis of proteasome interactions. Several proteasome-interacting proteins unique to synaptic 26S proteasomes, i.e. 14-3-3gamma, TAX1BP1, drebrin, SNAP-25, may modulate proteolysis in a synapse-specific manner. Three E3s, i.e. KCMF1, HUWE1, and UBE3A, and five DUBs, i.e. USP5, USP7, USP13, USP14, and UCH37, in association with synaptic proteasomes, which may help proteasomes function more efficiently, help determine specificity for certain types of conjugates, or insure the rapid elimination of ubiquitin chains released from the substrate | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus Sprague-Dawley | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | proteasome-mediated proteolysis is important for synaptic plasticity, neuronal development, protein quality control, and many other processes in neurons. The standard 26S subunits and a set of 28 proteasome-interacting proteins that associate substoichiometrically and may serve as regulators or cofactors in the brain differing in composition from other tissues. The content of proteasomes and their set of associated proteins can be altered by neuronal activity, in a manner likely to influence synaptic plasticity and learning | Rattus norvegicus |