Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bortezomib | an N-terminally blocked Phe-Leu dipeptide inhibitor containing a C-terminal boronic acid, inhibits chymotrypsin-likeand caspase-like actitivities of the proteasome, inhibitory pattern with the chymotrypsin-like activity of the intermediate-proteasome subtypes, overview | Rattus norvegicus | |
MG132 | i.e. Z-Leu-Leu-Leu-aldehyde, inhibits the chymotrypsin-like proteasome activity, inhibitory pattern with the chymotrypsin-like activity of the intermediate-proteasome subtypes, overview. Does not affect the caspase-like activity of subtypes II-2, I,-3, and I-4 at all | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Purification (Comment) | Organism |
---|---|
native 20S proteasomes from hearts by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | specific activities of the three standard proteasome subtypes II-2, II-3, and II-4 from rat heart exhibit high chymotrypsin-like activity, whereas the trypsin-like activity of these three subtypes account for only 1-2% compared with the chymotrypsin-like activity, their caspase-like activities overexceed the latter by 35-50%, overview | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-VGR-4-methyl-7-amido-coumarin + H2O | trypsin-like proteasome activity | Rattus norvegicus | benzoyl-VGR + 4-methyl-7-amino-coumarin | - |
? | |
N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O | chymotrypsin-like proteasome activity | Rattus norvegicus | N-succinyl-LLVY + 7-amino-4-methylcoumarin | - |
? | |
Z-LLE-4-methyl-7-amido-coumarin + H2O | post-acidic proteasome activity | Rattus norvegicus | Z-LLE + 4-methyl-7-amino-coumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
20S proteasome | - |
Rattus norvegicus |
proteasome | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |