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Literature summary for 3.4.25.1 extracted from

  • Djebali, W.; Gallusci, P.; Polge, C.; Boulila, L.; Galtier, N.; Raymond, P.; Chaibi, W.; Brouquisse, R.
    Modifications in endopeptidase and 20S proteasome expression and activities in cadmium treated tomato (Solanum lycopersicum L.) plants (2008), Planta, 227, 625-639.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
lactacystin 0.01 mM Solanum lycopersicum
N-acetyl-leucyl-leucyl-norleucinal MG132, 0.03 mM Solanum lycopersicum
PI 1 0.03 mM Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 0.3 mM Cd2+ lead to a 1.6 and 2.3fold increase in the 20S proteasome activity after 3 and 10 days, respectively, in leaves, the chymotrypsin activity of the 20S proteasome is maximally increased only after 3 days of treatment with 0.03 mM and 0.3 mM Cd2+ (1.5 and 2.5fold, respectively), no major effect of Cd2+ on the chymotrypsin activity of the 20S proteasome is observed in roots or leaves of plant treated with low Cd2+ concentrations (0.0003 mM and 0.003 mM) Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
550000
-
SDS-PAGE Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
variant Ibiza
-

Purification (Commentary)

Purification (Comment) Organism
Hiload 16/60 Superdex 200 gel filtration Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O substrate for chymotrypsin-like activity of the 20S proteasome Solanum lycopersicum ?
-
?

Synonyms

Synonyms Comment Organism
20S proteasome
-
Solanum lycopersicum