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Literature summary for 3.4.24.B4 extracted from

  • Zhang, X.; Wang, J.; Liu, M.; Wang, S.; Zhang, H.; Zhao, Y.
    Cloning, expression, purification, and characterization of the catalytic domain of sika deer MMP-13 (2016), Protein Expr. Purif., 127, 16-21 .
    View publication on PubMed
Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cervus nippon

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, no residual activity Cervus nippon
Cu2+ 10 mM, 15% residual activity Cervus nippon
EDTA 10 mM, 1% residual activity Cervus nippon
Fe2+ 10 mM, 9% residual activity Cervus nippon
Mg2+ 10 mM, 38% residual activity Cervus nippon
Mn2+ 10 mM, 2.5% residual activity Cervus nippon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 gelatin Km value is 0.136 mg/ml, pH 8.0, 37°C Cervus nippon

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ and Zn2+, absolutely required Cervus nippon
Zn2+ and Ca2+, absolutely required Cervus nippon

Organism

Organism UniProt Comment Textmining
Cervus nippon A0A142EHU4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, from inclusion bodies Cervus nippon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
collagen II + H2O complete degradation by the isolated catalytic domain Cervus nippon ?
-
 ?
Fibronectin + H2O partial degradation by the isolated catalytic domain Cervus nippon ?
-
 ?
Gelatin + H2O complete degradation by the isolated catalytic domain Cervus nippon ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, recombinant His-tagged catalytic domain Cervus nippon

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Cervus nippon

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, stable Cervus nippon
40
-
 30 min, about 80% los of activity Cervus nippon
50
-
 30 min, complete loss of activity Cervus nippon

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cervus nippon