Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CL-82198 | a specific MMP-13 inhibitor, inhibits also the metastatic activity of chloramphenicol-treated cells | Homo sapiens | |
additional information | no inhibition of MMP-13 by MMP-3 inhibitor N-isobutyl-N-[4-methoxyphenylsulphonyl] glycyl hydroxamic acid or by doxycycline | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Zn2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
GBM-8401 cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
Huh-7 cell | - |
Homo sapiens | - |
additional information | no MMP-13 expression in THP-1 cells, HL-60 cells, and Jurkat cells | Homo sapiens | - |
NCI-H1299 cell | - |
Homo sapiens | - |
NCI-N87 cell | - |
Homo sapiens | - |
SAS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
collagenase-3 | - |
Homo sapiens |
MMP-13 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | both the JNK inhibitor SP 600125 and PI-3K/Akt inhibitor LY 294002 can inhibit chloramphenicol-induced c-Jun phosphorylation, MMP-13 expression, and cell invasion | down |
Homo sapiens | chloramphenicol causes mitochondrial stress, decreases ATP biosynthesis, and induces MMP-13 expression, and solid-tumor cell invasion. Chloramphenicol also activated c-Jun N-terminal kinases, JNK, and PI-3K/Akt signaling, leading to c-Jun protein phosphorylation. The activated c-Jun protein activates binding to the MMP-13 promoter and also upregulates the amount of MMP-13. Other antibiotics that cause mitochondrial stress and a decrease in ATP biosynthesis also induce MMP-13 expression. Chloramphenicol-induction of MMP-13 expression is observed in nine solid-tumor cell lines, including H1299, HepG2, A549, CL-10, NCI-N87, Huh7, SAS, GBM8401, and Hep1, but not in leukemia suspensions, including THP-1, HL-60, and Jurkat cells | up |
General Information | Comment | Organism |
---|---|---|
additional information | chloramphenicol-induced MMP-13 expression is strongly associated with metastatic capacity of solid-tumor cells | Homo sapiens |