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Literature summary for 3.4.24.B4 extracted from

  • Shimizu, E.; Selvamurugan, N.; Westendorf, J.J.; Olson, E.N.; Partridge, N.C.
    HDAC4 represses matrix metalloproteinase-13 transcription in osteoblastic cells, and parathyroid hormone controls this repression (2010), J. Biol. Chem., 285, 9616-9626.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of a -148 rat MMP-13 promoter construct, subcloned upstream of a CAT reporter gene in pSV0, and of the mutant pGL2-MMP-13 promoter construct in UMR106-01 cells, real time quantitative RT-PCR expression analysis, overview Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information generation of a -148 rat MMP-13 promoter construct and a mutation construct of pGL2-MMP-13 promoter by site-directed mutagenesis. MMP-13 knockout by siRNA in UMR106-01 cells Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Mus musculus
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 wild-type and HDAC4-deficient mice
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Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
chondrocyte mineralizing hypertrophic Mus musculus
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femur
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 Mus musculus
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additional information MMP-13 mRNA abundance is greatly enhanced in Hdac4-deficient mice compared with wild-type or heterozygous littermates. MMP-13 staining in the tibiae of knockout mice is strongly detected in late hypertrophic chondrocytes and bone regions compared with wild-type or heterozygous mice Mus musculus
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osteoblast
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 Mus musculus
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osteoblast
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 Rattus norvegicus
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tibia
-
 Mus musculus
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UMR-106-01 cell
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 Rattus norvegicus
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Synonyms

Synonyms Comment Organism
MMP-13
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 Mus musculus
MMP-13
-
 Rattus norvegicus

Expression

Organism Comment Expression
Mus musculus HDAC4 represses MMP-13 transcription in osteoblastic cells, and parathyroid hormone controls this repression down
Rattus norvegicus HDAC4 represses MMP-13 transcription in osteoblastic cells, and parathyroid hormone controls this repression. HDAC4 interacts with Runx2 on the RD site of the MMP-13 promoter. Binding of HDAC4 to Runx2 is decreased after PTH stimulation down
Rattus norvegicus parathyroid hormone, PTH, stimulation of the MMP-13 promoter in the osteosarcoma cell line UMR-106-01, as well as in primary osteoblastic cells. Trichostatin A, an HDAC inhibitor, markedly stimulated basal transcription from the MMP-13 promoter in UMR 106-01 cells up