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Literature summary for 3.4.24.B29 extracted from

  • Hu, Y.; Peng, N.; Han, W.; Mei, Y.; Chen, Z.; Feng, X.; Liang, Y.X.; She, Q.
    An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease (2012), Biosci. Rep., 32, 609-618.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
leupeptin 1 mM, 1.7fold activation Saccharolobus solfataricus
PMSF 5 mM, 1.2fold activation Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C416G 4% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
E229D 11% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
H228F 8% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
H233Y 14% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, 90% inhibition Saccharolobus solfataricus
Co2+ 5 mM, 91% inhibition Saccharolobus solfataricus
Cu2+ 5 mM, 37% inhibition Saccharolobus solfataricus
E-64 0.01 mM, 44% inhibition Saccharolobus solfataricus
EDTA 5 mM, complete inhibition Saccharolobus solfataricus
Fe2+ 5 mM, 93% inhibition Saccharolobus solfataricus
Hg2+ 5 mM, 15% inhibition Saccharolobus solfataricus
Mg2+ 5 mM, 97% inhibition Saccharolobus solfataricus
Mn2+ 5 mM, 37% inhibition Saccharolobus solfataricus
N-ethylmaleimide 5 mM, 64% inhibition Saccharolobus solfataricus
Sr2+ 5 mM, 83% inhibition Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 5 mM, stimulates activity up to 160%, contains a zinc-binding motif Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q7LXP6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Saccharolobus solfataricus fragments of azocasein
-
?

Subunits

Subunits Comment Organism
dimer 2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
Sso0660
-
Saccharolobus solfataricus
TldD
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 85 45°C: about 85% of maximal activity, 85°C: about 60% of maximal activity Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 6: more than 75% of maximal activity, pH 9: about 80% of maximal activity Saccharolobus solfataricus