Activating Compound | Comment | Organism | Structure |
---|---|---|---|
leupeptin | 1 mM, 1.7fold activation | Saccharolobus solfataricus | |
PMSF | 5 mM, 1.2fold activation | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C416G | 4% of the activity compared to wild-type enzyme, azocasein as substrate | Saccharolobus solfataricus |
E229D | 11% of the activity compared to wild-type enzyme, azocasein as substrate | Saccharolobus solfataricus |
H228F | 8% of the activity compared to wild-type enzyme, azocasein as substrate | Saccharolobus solfataricus |
H233Y | 14% of the activity compared to wild-type enzyme, azocasein as substrate | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 10 mM, 90% inhibition | Saccharolobus solfataricus | |
Co2+ | 5 mM, 91% inhibition | Saccharolobus solfataricus | |
Cu2+ | 5 mM, 37% inhibition | Saccharolobus solfataricus | |
E-64 | 0.01 mM, 44% inhibition | Saccharolobus solfataricus | |
EDTA | 5 mM, complete inhibition | Saccharolobus solfataricus | |
Fe2+ | 5 mM, 93% inhibition | Saccharolobus solfataricus | |
Hg2+ | 5 mM, 15% inhibition | Saccharolobus solfataricus | |
Mg2+ | 5 mM, 97% inhibition | Saccharolobus solfataricus | |
Mn2+ | 5 mM, 37% inhibition | Saccharolobus solfataricus | |
N-ethylmaleimide | 5 mM, 64% inhibition | Saccharolobus solfataricus | |
Sr2+ | 5 mM, 83% inhibition | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 5 mM, stimulates activity up to 160%, contains a zinc-binding motif | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q7LXP6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Saccharolobus solfataricus | fragments of azocasein | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
Sso0660 | - |
Saccharolobus solfataricus |
TldD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 85 | 45°C: about 85% of maximal activity, 85°C: about 60% of maximal activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 6: more than 75% of maximal activity, pH 9: about 80% of maximal activity | Saccharolobus solfataricus |