Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B19 extracted from

  • Weber, E.R.; Hanekamp, T.; Thorsness, P.E.
    Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S. cerevisiae (1996), Mol. Biol. Cell, 7, 307-317.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B19

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme in Escherichia coli strain DH5alpha, expression of deletion mutant in Escherichia coli strain BMH 71-18 mut S Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of inactive mutant by site-directed mutagenesis in the ATP and Zn2+ binding domains, inactivation of the enzyme causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
deoxycholate
-
 Saccharomyces cerevisiae
proteinase K
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Saccharomyces cerevisiae 5743
-
mitochondrion tightly associated with the inner mitochondrial membrane, oriented with the bulk of the protein facing the matrix Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on, 2 zinc ligands are the conserved His540 and His546 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
unassembled gamma subunit of mitochondrial ATP-synthase + H2O Saccharomyces cerevisiae i.e. Atp3p ?
-
 ?
unassembled subunit II of cytochrome oxidase + H2O Saccharomyces cerevisiae i.e. Cox2p ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
unassembled gamma subunit of mitochondrial ATP-synthase + H2O i.e. Atp3p Saccharomyces cerevisiae ?
-
 ?
unassembled subunit II of cytochrome oxidase + H2O i.e. Cox2p Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
AAA protease
-
 Saccharomyces cerevisiae
M41.004 Merops-ID Saccharomyces cerevisiae
Yme1p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP required, contains ATP-binding site spanning from residues 316 to 328, GVLLTGPPGTGKT Saccharomyces cerevisiae