Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimal aggrecanase activity for ADAMTS-5 is detected around 200 mM NaCl | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in HEK-293-EBNA cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by N-TIMP-1 or TIMP-2; the general proteolytic activitiy of ADAMTS-5 as measured with carboxymethylated transferrin is unaffected by concentrations up to 1 M NaCl | Homo sapiens | |
NaCl | the activity is very low at or below 50 mM NaCl or above 500 mM NaCl | Homo sapiens | |
TIMP-3 | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a zinc catalytic domain | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
M2-agarose column chromatography, Macro-Prep S resin column chromatography, and Sephacryl S-200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | - |
Homo sapiens | - |
SW-1353 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aggrecan + H2O | ADAMTS-5 is able to cleave aggrecan core protein about 1,000 times more effectively than ADAMTS-4 | Homo sapiens | ? | - |
? | |
biglycan + H2O | - |
Homo sapiens | ? | - |
? | |
carboxymethylated transferrin + H2O | - |
Homo sapiens | ? | - |
? | |
casein + H2O | - |
Homo sapiens | ? | - |
? | |
decorin + H2O | - |
Homo sapiens | ? | - |
? | |
fibromodulin + H2O | very low activity | Homo sapiens | 29000 Da fibromodulin fragment + ? | - |
? | |
Fibronectin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | weak activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adamalysin with thrombospondin type 1 motifs-5 | - |
Homo sapiens |
ADAMTS-5 | - |
Homo sapiens |
aggrecanase 2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Homo sapiens |