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Literature summary for 3.4.24.B12 extracted from

  • Gendron, C.; Kashiwagi, M.; Lim, N.H.; Enghild, J.J.; Thogersen, I.B.; Hughes, C.; Caterson, B.; Nagase, H.
    Proteolytic activities of human ADAMTS-5: comparative studies with ADAMTS-4 (2007), J. Biol. Chem., 282, 18294-18306.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NaCl optimal aggrecanase activity for ADAMTS-5 is detected around 200 mM NaCl Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293-EBNA cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by N-TIMP-1 or TIMP-2; the general proteolytic activitiy of ADAMTS-5 as measured with carboxymethylated transferrin is unaffected by concentrations up to 1 M NaCl Homo sapiens
NaCl the activity is very low at or below 50 mM NaCl or above 500 mM NaCl Homo sapiens
TIMP-3
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains a zinc catalytic domain Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
M2-agarose column chromatography, Macro-Prep S resin column chromatography, and Sephacryl S-200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cartilage
-
Homo sapiens
-
SW-1353 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O ADAMTS-5 is able to cleave aggrecan core protein about 1,000 times more effectively than ADAMTS-4 Homo sapiens ?
-
?
biglycan + H2O
-
Homo sapiens ?
-
?
carboxymethylated transferrin + H2O
-
Homo sapiens ?
-
?
casein + H2O
-
Homo sapiens ?
-
?
decorin + H2O
-
Homo sapiens ?
-
?
fibromodulin + H2O very low activity Homo sapiens 29000 Da fibromodulin fragment + ?
-
?
Fibronectin + H2O
-
Homo sapiens ?
-
?
Gelatin + H2O weak activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
adamalysin with thrombospondin type 1 motifs-5
-
Homo sapiens
ADAMTS-5
-
Homo sapiens
aggrecanase 2
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9.5
-
Homo sapiens