Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells via baculovirus infection, secretion of recombinant protein to the medium | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | - |
Bos taurus | |
heparin | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
extracellular | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
aggrecan + H2O | Homo sapiens | enzyme may play a role in cartilage breakdown | ? | - |
? | |
aggrecan + H2O | Bos taurus | enzyme may play a role in cartilage breakdown | ? | - |
? | |
proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
proteins + H2O | Bos taurus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q9TT92 | - |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage by furin | Homo sapiens |
proteolytic modification | cleavage by furin | Bos taurus |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged from insect Sf9 cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | - |
Homo sapiens | - |
synovial membrane | - |
Bos taurus | - |
synovium | - |
Bos taurus | - |
synovium | arthritic | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aggrecan + H2O | substrate from cartilage, extensive digestion, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and between chondroitin sulfate attachment sites 1 and 2 E1480-G | Homo sapiens | ? | - |
? | |
aggrecan + H2O | substrate from cartilage, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and within the chondroitinsulfate 2 attachment region E1666-G, E1771-A, and E1871-L | Bos taurus | ? | - |
? | |
aggrecan + H2O | enzyme may play a role in cartilage breakdown | Homo sapiens | ? | - |
? | |
aggrecan + H2O | enzyme may play a role in cartilage breakdown | Bos taurus | ? | - |
? | |
additional information | interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzyme | Bos taurus | ? | - |
? | |
additional information | interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzymew | Homo sapiens | ? | - |
? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
proteins + H2O | - |
Bos taurus | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS-5 | - |
Homo sapiens |
ADAMTS-5 | - |
Bos taurus |
aggrecanase-2 | - |
Homo sapiens |
aggrecanase-2 | - |
Bos taurus |
M12.225 | Merops-ID | Homo sapiens |
M12.225 | Merops-ID | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Bos taurus |