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Literature summary for 3.4.24.B10 extracted from

  • Loechel, F.; Gilpin, B.J.; Engvall, E.; Albrechtsen, R.; Wewer, U.M.
    Human ADAM 12 (meltrin alpha) is an active metalloprotease (1998), J. Biol. Chem., 273, 16993-16997.
    View publication on PubMed
Search for more literature for 3.4.24.B10

Cloned(Commentary)

Cloned (Comment) Organism
transient expression in COS-7 cells of an enzyme mutant where the signal peptide, prodomain and metalloprotease domain are exchanged for an Ig kappa-chain leader sequence, secretion to the medium Homo sapiens

Protein Variants

Protein Variants Comment Organism
C179A site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain results in a no longer latent but inactive zymogen Homo sapiens
C179S site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain results in a no longer latent but inactive zymogen Homo sapiens
E351Q site-directed mutagenesis, exchange of the glutamic acid residue of the zinc binding domain involved in catalytic activity, inactive to form complexes with alpha2-macroglobulin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
EDTA complete inhibition Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ highly conserved zinc-binding motif HEXXHXXGXXH, the metalloprotease domain is the catalytically active site, the Met-turn is essential for the structural integrity of the zinc-binding site, Glu351 is essential for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE Homo sapiens
92000
-
 x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme complexes alpha2-macroglobulin ?
-
 ?
proteins + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43184
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the cysteine-switch of the prodomain maintaines the zymogen in a latent state, enzyme is synthesized as a catalytically inactive zymogen, in vivo cleavage by a furin-like endopeptidase, activation is chemically possible with N-ethylmaleimide, but not with Hg2+ Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolysis of proteins the metalloprotease domain is catalytically active, Glu351 is essential for activity Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme complexes alpha2-macroglobulin Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
proteins + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
a disintegrin and metalloproteinase domain 12
-
 Homo sapiens
ADAM12
-
 Homo sapiens
M12.212 Merops-ID Homo sapiens
meltrin alpha
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 alpha2-macroglobulin complexing assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 alpha2-macroglobulin complexing assay at Homo sapiens