Cloned (Comment) | Organism |
---|---|
expression of the soluble enzyme form ADAM 12-S in COS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C179S | site-directed mutagenesis, mutant behaves identical to the wild-type concerning metal ions, therfore the cysteine-switch and activation by copper are 2 different components of enzyme activation | Homo sapiens |
additional information | elimination of the cysteine-switch by a point mutation in the propeptide has no effect on copper activation, whereas mutation of an unpaired cysteine residue in the catalytic domain resulted in a mutant form of ADAM 12-S that is insensitiv to copper | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates, dependent on, multistep activation mechanism involving both furin cleavage and copper binding | Homo sapiens | |
Zn2+ | activates, dependent on, enzyme contains a catalytically active zinc-binding domain | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43184 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | autocleavage occurs near the junction of the prodomain and the catalytic domain upon inactivation of cysteine residues C179 and in presence of Cu2+ which is further promoted in presence of ascorbate, multistep activation mechanism involving both furin cleavage and copper binding | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant from COS cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolysis of proteins | active site zinc ion is coordinated by Cys179 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | - |
Homo sapiens | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
a disintegrin and metalloprotease-12 | - |
Homo sapiens |
ADAM 12-S | - |
Homo sapiens |
M12.212 | Merops-ID | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |