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Literature summary for 3.4.24.B10 extracted from

  • Loechel, F.; Fox, J.W.; Murphy, G.; Albrechtsen, R.; Wewer, U.M.
    ADAM 12-S cleaves IGFBP-3 and IGFBP-5 and is inhibited by TIMP-3 (2000), Biochem. Biophys. Res. Commun., 278, 511-515.
    View publication on PubMed
Search for more literature for 3.4.24.B10

Activating Compound

Activating Compound Comment Organism Structure
additional information IGF-I and IGF-II are not stimulating, the enzyme is IGF-independent Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the soluble secreted enzyme form ADAM 12-S in cell line 293-EBNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-[[[6-cyano-1-[(1-methyl-1H-imidazol-5-yl)methyl]-1,2,3,4,6,7-hexahydroquinolin-3-yl](pyridin-2-ylsulfonyl)amino]methyl]-N,N-dimethylpiperidine-1-carboxamide
-
 Homo sapiens
tissue inhibitor of metalloproteinase-3 i.e. TIMP-3, possible physiological inhibitor of the enzyme Homo sapiens
Zn2+ at 0.1 mM complete inhibition Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular blood Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloprotease Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 x * 27000, prodomain after cleavage of the proform with furin, SDS-PAGE, x * 68000, recombinant processed enzyme form, SDS-PAGE, x * 92000, recombinant soluble enzyme proform, SDS-PAGGE Homo sapiens
68000
-
 x * 27000, prodomain after cleavage of the proform with furin, SDS-PAGE, x * 68000, recombinant processed enzyme form, SDS-PAGE, x * 92000, recombinant soluble enzyme proform, SDS-PAGGE Homo sapiens
92000
-
 x * 27000, prodomain after cleavage of the proform with furin, SDS-PAGE, x * 68000, recombinant processed enzyme form, SDS-PAGE, x * 92000, recombinant soluble enzyme proform, SDS-PAGGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
insulin-like growth factor binding protein-3 + H2O Homo sapiens enzyme plays a role in degradation of insulin-like growth factor binding protein-3 in the blood of pregnant women ?
-
 ?
insulin-like growth factor binding protein-5 + H2O Homo sapiens
-
 ?
-
 ?
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O43184
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from cell line 293-EBNA Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood of pregnant women Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insulin-like growth factor binding protein-3 + H2O
-
 Homo sapiens ?
-
 ?
insulin-like growth factor binding protein-3 + H2O enzyme plays a role in degradation of insulin-like growth factor binding protein-3 in the blood of pregnant women Homo sapiens ?
-
 ?
insulin-like growth factor binding protein-5 + H2O
-
 Homo sapiens ?
-
 ?
additional information no activity with insulin-like growth factor binding proteins-1, -2, -4 and -6 Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, prodomain after cleavage of the proform with furin, SDS-PAGE, x * 68000, recombinant processed enzyme form, SDS-PAGE, x * 92000, recombinant soluble enzyme proform, SDS-PAGGE Homo sapiens

Synonyms

Synonyms Comment Organism
ADAM12
-
 Homo sapiens
M12.212 Merops-ID Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 enzyme is active at neutral and alkaline pH, but inactive below pH 6.5 Homo sapiens