Literature summary for 3.4.24.87 extracted from

Akiyama, M.; Takeda, S.; Kokame, K.; Takagi, J.; Miyata, T.
Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor (2009), Proc. Natl. Acad. Sci. USA, 106, 19274-19279.

Search for more literature for 3.4.24.87

Cloned(Commentary)

Cloned (Comment)	Organism
expression of structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences in CHO Lec 3.2.8.1 cells. Transient expression of His-tagged mutant ADAMTS13-MDTCS domains using a cytomegalovirus promoter-driven expression vector in HeLa cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant detagged ADAMTS13-DTCS, residues 287-685, an non-catalytic von Willebrand factor-exosite-containing ADAMTS13 fragment, sitting drop vapor diffusion method, mixing of 500 nl of protein solution and 500 nl reservoir solution containing 26% w/v PEG1500, 100 mM MES, pH 6.0, supplemented with 200 nl of 40% w/w pentaerythritol ethoxylate, equilibration for several days at 20°C. Os-derivative crystals by soaking native crystals in reservoir solution supplemented with 1 mM OsCl3 and 20% glycerol for several hours, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution resulting in two crystal structures	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant detagged ADAMTS13-DTCS, residues 287-685, an non-catalytic von Willebrand factor-exosite-containing ADAMTS13 fragment, sitting drop vapor diffusion method, mixing of 500 nl of protein solution and 500 nl reservoir solution containing 26% w/v PEG1500, 100 mM MES, pH 6.0, supplemented with 200 nl of 40% w/w pentaerythritol ethoxylate, equilibration for several days at 20°C. Os-derivative crystals by soaking native crystals in reservoir solution supplemented with 1 mM OsCl3 and 20% glycerol for several hours, X-ray diffraction structure determination and analysis at 2.6 A and 2.8 A resolution resulting in two crystal structures

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of structure-based mutants of ADAMTS13-MDTCS residues 75685 fragment. The MDTCS domains are conserved among ADAMTS family proteins	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	MDTCS domain modelling and substrate recognition mode analysis, overview	?	-	?
von Willebrand factor + H2O	Homo sapiens	specific for	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant structure-based mutants of ADAMTS13-MDTCS residues 75-685 fragment with a C-terminal tobacco etch virus proteinase cleavage site followed by tandem His-tag sequences from CHO Lec 3.2.8.1 cells by nickel affinity chromatography and gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
plasma	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
FRETS-VWF73 peptide + H2O	fluorogenic von Willebrand factor-derived peptide substrate	Homo sapiens	?	-	?
additional information	MDTCS domain modelling and substrate recognition mode analysis, overview	Homo sapiens	?	-	?
von Willebrand factor + H2O	specific for	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	ADAMTS13-DTCS, residues 287-685, an exosite-containing ADAMTS13 fragment, structures reveal folding similarities between the disintegrin-like domain and the N-terminal portion of the cysteine-rich domain. The spacer domain forms a globular functional unit with a 10-stranded-sandwich fold that has multiple interaction sites with the cysteine-rich domain. The MDTCS domains are conserved among ADAMTS family proteins	Homo sapiens

Synonyms

Synonyms	Comment	Organism
ADAMTS13	ADAMTS13 belongs to the ADAMTS or a disintegrin-like and metalloproteinase with thrombospondin type-1 motif family	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	ADAMTS13 specifically cleaves plasma von Willebrand factor and thereby controls von Willebrand factor-mediated platelet thrombus formation. Severe deficiencies in ADAMTS13 can cause life-threatening thrombotic thrombocytopenic purpura	Homo sapiens