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Literature summary for 3.4.24.87 extracted from

  • Fujikawa, K.; Suzuki, H.; McMullen, B.; Chung, D.
    Purification of human von Willebrand factor-cleaving protease and its identification as a new member of the metalloproteinase family (2001), Blood, 98, 1662-1666.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Urea activation, substrate is degraded at 1 M Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular plasma Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on Homo sapiens
additional information enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
1 * 150000, SDS-PAGE Homo sapiens
150000 190000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
proteins + H2O Homo sapiens
-
peptides
-
?
von Willebrand factor + H2O Homo sapiens cleavage of peptide bond Tyr842-Met843 2 peptides of 140 kD and 65 kD
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif
-

Purification (Commentary)

Purification (Comment) Organism
from commercial factor VIII/vWF concentrate Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
The enzyme cleaves the von Willebrand factor at bond Tyr842-/-Met843 within the A2 domain active site sequence HEIGHSFGLEHE 150 amino acid residues from the N-terminus of the protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Homo sapiens
-
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
proteins + H2O
-
Homo sapiens peptides
-
?
von Willebrand factor + H2O cleavage of peptide bond Tyr842-Met843 Homo sapiens 2 peptides of 140 kD and 65 kD
-
?

Subunits

Subunits Comment Organism
monomer 1 * 150000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
M12.241 Merops-ID Homo sapiens
vWF-cleaving protease
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens