Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Urea | activation, substrate is degraded at 1 M | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | plasma | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Homo sapiens | |
additional information | enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
1 * 150000, SDS-PAGE | Homo sapiens |
150000 | 190000 | gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
von Willebrand factor + H2O | Homo sapiens | cleavage of peptide bond Tyr842-Met843 | 2 peptides of 140 kD and 65 kD | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
enzyme is a member of the ADAMTS metalloprotease family, disintegrin and metalloproteinase with thrombospondin type I motif | - |
Purification (Comment) | Organism |
---|---|
from commercial factor VIII/vWF concentrate | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
The enzyme cleaves the von Willebrand factor at bond Tyr842-/-Met843 within the A2 domain | active site sequence HEIGHSFGLEHE 150 amino acid residues from the N-terminus of the protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
von Willebrand factor + H2O | cleavage of peptide bond Tyr842-Met843 | Homo sapiens | 2 peptides of 140 kD and 65 kD | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 150000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
M12.241 | Merops-ID | Homo sapiens |
vWF-cleaving protease | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |