Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
E687D | mutation in metal-binding site, decrease in catalytic activity | Bacillus anthracis |
H690A | mutation in metal-binding site, decrease in catalytic activity | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
fluorescein-QRRKKVYPYPME | wild-type, pH 7.4, 37°C | Bacillus anthracis | |
0.0174 | - |
fluorescein-QRRKKVYPYPME | mutant E687D, pH 7.4, 37°C | Bacillus anthracis | |
0.042 | - |
fluorescein-QRRKKVYPYPME | mutant H690A, pH 7.4, 37°C | Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation ability of divalent ions decreases in the follwing order: Zn2+ Ca2+ Mn2+ Mg2+, with Mg2+ completely unable to activate the enzyme | Bacillus anthracis | |
Mn2+ | activation ability of divalent ions decreases in the follwing order: Zn2+ Ca2+ Mn2+ Mg2+, with Mg2+ completely unable to activate the enzyme | Bacillus anthracis | |
additional information | Mg2+ is unable to activate | Bacillus anthracis | |
Zn2+ | activation ability of divalent ions decreases in the follwing order: Zn2+ Ca2+ Mn2+ Mg2+, with Mg2+ completely unable to activate the enzyme | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Preferred amino acids around the cleavage site can be denoted BBBBxHx-/-H, in which B denotes Arg or Lys, H denotes a hydrophobic amino acid, and x is any amino acid. The only known protein substrates are mitogen-activated protein (MAP) kinase kinases | pre-steady-state kinetics of anthrax lethal factor proteolysis follows a four-step mechanism as follows: initial substrate binding, rearrangement of the enzyme-substrate complex, a rate-limiting cleavage step, and product release | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-RDIRRITLFSLH | i.e. S20D, substrate isolated from phage library | Bacillus anthracis | ? | - |
? | |
fluorescein-QRRKKVYPYPME + H2O | i.e. LF15, peptide substrate isolated from second-iteration substrate phage library | Bacillus anthracis | fluorescein-QRRKKVYP + YPME | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
fluorescein-QRRKKVYPYPME | mutant H690A, pH 7.4, 37°C | Bacillus anthracis | |
0.1 | - |
fluorescein-QRRKKVYPYPME | mutant E687D, pH 7.4, 37°C | Bacillus anthracis | |
0.52 | - |
fluorescein-QRRKKVYPYPME | wild-type, pH 7.4, 37°C | Bacillus anthracis |