Application | Comment | Organism |
---|---|---|
molecular biology | results suggest that this toxin delivery system is capable of stimulating protective immune responses where effective immunization requires stimulation of both classes of T cells | Bacillus anthracis |
Cloned (Comment) | Organism |
---|---|
recombinant anthrax lethal toxin proteins consisting a model CD4 T-cell epitope from chicken ovalbumin (Ova) fused to nontoxic lethal factor (LFn). The antigen tags are generated by annealing single-stranded DNA oligonucleotides to form double-stranded DNA Plasmids are transformed into Escherichia coli BL21 | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in order to examine the role of protective antigen a lethal factor (LFn) fusion protein bearing two epitopes from Ova, one restricted by MHC-II and one restricted by MHC-I is generated. This single LFn fusion protein is capable of stimulating both ovalbumin-specific CD4+ and ovalbumin-specific CD8+ T-cell responses in mice | Bacillus anthracis |
additional information | it is investigated whether lethal factor (LFn) fusion proteins and protective antigen can also be used to deliver antigen to the MHC-II pathway for the stimulation of antigen-specific CD4+ T-cells. A CD4+ T-cell epitope from chicken ovalbumin is fused to nontoxic LFn and demonstrates that this recombinant protein induces an ovalbumin-specific CD4+ T-cell response both in vitro and in mice | Bacillus anthracis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the recombinant LFn fusion proteins contain a vector-encoded His6 tag at the amino terminus and are purified by Ni2+-affinity chromatography to greater than 95% purity as determined by Coomassie staining | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
anthrax lethal toxin | - |
Bacillus anthracis |
lethal factor | lethal toxin is secreted by Bacillus anthracis as a bipartite toxin consisting of protective antigen (PA) and lethal factor (LF) | Bacillus anthracis |