Literature summary for 3.4.24.81 extracted from

Escrevente, C.; Morais, V.A.; Keller, S.; Soares, C.M.; Altevogt, P.; Costa, J.
Functional role of N-glycosylation from ADAM10 in processing, localization and activity of the enzyme (2008), Biochim. Biophys. Acta, 1780, 905-913.

Search for more literature for 3.4.24.81

Cloned(Commentary)

Cloned (Comment)	Organism
a C-terminal HA-tagged fusion protein is transfected in human ovarian cancer SKOV3 cells and human embryonic kidney HEK293 cells	Homo sapiens
a C-terminal HA-tagged fusion protein is transfected in human ovarian cancer SKOV3 cells and human embryonic kidney HEK293 cells	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
S269A	mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied	Bos taurus
S441A	mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied, mutant shows increased ADAM10 susceptibility to proteolysis	Bos taurus
T280A	mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied, T280A is found to accumulate in the endoplasmic reticulum as the non-processed precursor of the enzyme. Mutant exhibits only residual levels of metalloprotease activity	Bos taurus
T553A	mutant is detected at lower molecular masses than the wild-type form, which indicates that the N-glycosylation site is occupied	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome	-	Bos taurus	5768	-
perinuclear space	predominant localization of the protein at the perinuclear region and at the cell surface where it appears as punctuated dots evenly distributed at the plasma membrane	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	Western blot, non-reducing conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	contained high-mannose as well as complex-type N-glycans	Homo sapiens
glycoprotein	contains high-mannose as well as complex-type N-glycans. Glycosylation sites: S269, T280, S441, T553	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
SKOV-3 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dap(dnp)-NH2 + H2O	-	Homo sapiens	?	-	?
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dap(dnp)-NH2 + H2O	-	Bos taurus	?	-	?
L1 cell adhesion molecule + H2O	-	Bos taurus	?	-	?

Synonyms

Synonyms	Comment	Organism
ADAM10	-	Homo sapiens
ADAM10	-	Bos taurus

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Release 2023.1 (January 2023)