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Literature summary for 3.4.24.80 extracted from

  • Majkowska, I.; Shitomi, Y.; Ito, N.; Gray, N.S.; Itoh, Y.
    Discoidin domain receptor 2 mediates collagen-induced activation of membrane-type 1 matrix metalloproteinase in human fibroblasts (2017), J. Biol. Chem., 292, 6633-6643 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.80

Activating Compound

Activating Compound Comment Organism Structure
additional information collagen I up-regulates both MT1-MMP gene and functions in various cell types Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information specific enzyme knockout by MT1-MMP siRNA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DX-2400
-
 Homo sapiens
GM6001
-
 Homo sapiens
TIMP-2
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-
membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen I + H2O Homo sapiens
-
 ?
-
 ?
Gelatin + H2O Homo sapiens
-
 ?
-
 ?
proMMP-2 + H2O Homo sapiens
-
 MMP-2 + MMP-2 pro-peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50281
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification discoidin domain receptor 2 (DDR2) mediates collagen I-induced activation of membrane-type 1 matrix metalloproteinase in human fibroblasts Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
 Homo sapiens
-
carcinoma cell invading Homo sapiens
-
fibroblast
-
 Homo sapiens
-
fibrosarcoma cell
-
 Homo sapiens
-
HT-1080 cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
additional information MT1-MMP is highly expressed in cells with invading capacity, including fibroblasts and invasive cancer cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
collagen I + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
proMMP-2 + H2O
-
 Homo sapiens MMP-2 + MMP-2 pro-peptide
-
 ?

Synonyms

Synonyms Comment Organism
membrane-type 1 matrix metalloproteinase
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens collagen upregulates both MT1-MMP gene and functions in various cell types up

General Information

General Information Comment Organism
additional information DDRs are receptor tyrosine kinases (RTKs) in which phosphorylation of their cytoplasmic domain is induced by binding to collagen at their ectodomain. Discoidin domain receptor 2 (DDR2) mediates type I collagen-induced activation of membrane-type 1 matrix metalloproteinase in human fibroblasts. Knocking down DDR2, but not the beta1 integrin subunit, a common subunit for all collagen-binding integrins, inhibits the collagen-induced MT1-MMP-dependent activation of pro-MMP-2 and upregulation of MT1-MMP at the gene and protein levels. This DDR2-mediated mechanism is only present in non-transformed mesenchymal cells, as collagen-induced MT1-MMP activation in HT-1080 fibrosarcoma cells and MT1-MMP function in MDA-MB231 breast cancer cells are not affected by DDR kinase inhibition Homo sapiens
physiological function the cell-surface collagen degradation by MT1-MMP involves DDR2-mediated collagen signaling Homo sapiens