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Literature summary for 3.4.24.80 extracted from

  • Itoh, Y.; Takamura, A.; Ito, N.; Maru, Y.; Sato, H.; Suenaga, N.; Aoki, T.; Seiki, M.
    Homophilic complex formation of MT1-MMP facilitates proMMP-2 activation on the cell surface and promotes tumor cell invasion (2001), EMBO J., 20, 4782-4793.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.80

Activating Compound

Activating Compound Comment Organism Structure
additional information the PEX domain is required for activating cleavage activity on substrate proMMP-2 on the cell surface Homo sapiens
TIMP-2 required, MT-MMP1 must form a homophilic ternary complex with TIMP-2 and pro-MMP-2 to activate MMP-2 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the truncation mutant MT1EADELTATM in Escherichia coli, expression of the PEX-domain-deleted MT1-MMP mutant Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of truncation mutant MT1EADELTATM lacking the pro-peptide and the transmembrane and cytoplasmic domains. Mutant MT1EADELTATM shows different mobility in reducing and non-reducing PAGE compared to the wild-type enzyme, the disulfide bond in the PEX domain is correctly folded, there is no intermolecular disulfide bond formation, and the complex with TIMP-2 is also correctly formed. A PEX-domain-deleted MT1-MMP mutant is no longer able to activate proMMP-2, but retains some proteolytic activity and forms the ternery complex with proMMP-2 and TIMP-2. A MT1-MMP mutant lacking the catalytic domain is catalytically inactive and inhibits the dimerization and complex formation of the enzyme, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Rac1 inhibits both enzyme activity and dimerization Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface MT1-MMP forms a homophilic complex required for activity Homo sapiens 9986
-
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 monomeric mutant MT1EADELTATM, native PAGE Homo sapiens
130000
-
 dimeric mutant MT1EADELTATM, native PAGE Homo sapiens
600000
-
 oligomeric mutant MT1EADELTATM, native PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-MMP-2 + H2O Homo sapiens MT-MMP1 activates MMP-2, EC 3.4.24.24 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HT-1080 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-MMP-2 + H2O MT-MMP1 activates MMP-2, EC 3.4.24.24 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 50000, mutant MT1EADELTATM, SDS-PAGE Homo sapiens
monomer 1 * 50000, mutant MT1EADELTATM, SDS-PAGE Homo sapiens
More dimer formation is specific to the PEX domain of MT1-MMP, the PEX domain of MT4-MMP does not substitute for it Homo sapiens
oligomer 1 * 50000, mutant MT1EADELTATM, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MT-MMP1
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function MT-MMP1 must form a homophilic ternary complex with TIMP-2 and pro-MMP-2 to activate MMP-2 Homo sapiens