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Literature summary for 3.4.24.80 extracted from

  • Cao, J.; Kozarekar, P.; Pavlaki, M.; Chiarelli, C.; Bahou, W.F.; Zucker, S.
    Distinct roles for the catalytic and hemopexin domains of membrane type 1-matrix metalloproteinase in substrate degradation and cell migration (2004), J. Biol. Chem., 279, 14129-14139.
    View publication on PubMed
Search for more literature for 3.4.24.80

Application

Application Comment Organism
medicine MT1-MMP initiates substrate degradation and enhances cell migration, hemopexin-like domain and a nonenzymatic component of the catalytic domain of MT1-MMP are essential for MT1-MMP-mediated cell migration, Rac1 participates in the MT1-MMP signal transduction pathway Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pSG5 vector, expression in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E240A expressed on transfected cell surface but fails to activate pMMP-2 Homo sapiens
K44A dominant negative dynamin mutation controlled by a separate cytomegalovirus promoter MT1/K44A, leading to increased substrate digestion that is contributed by enhanced cell migration resulting from the accumulation of MT1-MMP ant the plasma membrane Homo sapiens
additional information mutant Sol.MT, fails to activate pMMP-2, mutant MTdeltaC, pMMP-2 activation as compared with wild type, neither cell type-dependent nor extracellular matrix component-dependent Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information cells cotransfected with TIMP-1 cDNA along with MT1-MMP and pMMP-2 cDNAs result in partial inhibition of substrate degradation, down to the basal digestion level resulting from MT1-MMP alone, wortmannin and PD98059 do not interfer with MT1-MMP-induced cell migration, CDC42 (N17) and RhoA(N19) have no effect on MT1-MMP-dependent cell migration Homo sapiens
Rac1(N17Rac) coexpression wit MT1-MMP cDNAs leads to complete inhibition of migration Homo sapiens
TIMP-2 totally abolishes substrate degradation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm short cytoplasmic tail Homo sapiens 5737
-
perinuclear space highest amounts Homo sapiens
-
-
plasma membrane plasma membrane anchor Homo sapiens 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 MT1-GFP fusion protein, Western blot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell line
-
 Homo sapiens
-
LNCaP cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
prostate cancer cell line
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibrin + H2O
-
 Homo sapiens ?
-
 ?
Fibrinogen + H2O
-
 Homo sapiens ?
-
 ?
Fibronectin + H2O cell surface active pMMP-2 cDNAs bound to MT1-MMP enhances substrate digestion, cytoplasmic tail of MT1-MMP not required for digestion Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
membrane type 1 matrix metalloproteinase
-
 Homo sapiens
MT1-MMP
-
 Homo sapiens