Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.72 extracted from

  • Bi, Q.; Han, B.; Liu, W.; Feng, Y.; Jiang, Z.
    UFEIII, a fibrinolytic protease from the marine invertebrate, Urechis unicinctus (2013), Biotechnol. Lett., 35, 1115-1120.
    View publication on PubMed
Search for more literature for 3.4.24.72

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 29% residual activity at 5 mM Urechis unicinctus
Cu2+ 32% residual activity at 5 mM Urechis unicinctus
EDTA 45% residual activity at 1 mM Urechis unicinctus
EGTA 36% residual activity at 1 mM Urechis unicinctus
Fe2+ 70% residual activity at 5 mM Urechis unicinctus
Fe3+ 36% residual activity at 5 mM Urechis unicinctus
phenylmethylsulfonyl fluoride 28% residual activity at 4 mM Urechis unicinctus
protein SBTI 26% residual activity at 4 mM Urechis unicinctus
Zn2+ 42% residual activity at 5 mM Urechis unicinctus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 118% activity at 5 mM Urechis unicinctus
Mn2+ 118% activity at 5 mM Urechis unicinctus
additional information not influenced by Ca2+ Urechis unicinctus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20800
-
 1 * 20800, SDS-PAGE Urechis unicinctus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrinogen + H2O Urechis unicinctus
-
 fibrin + ?
-
 ?
plasminogen + H2O Urechis unicinctus
-
 plasmin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Urechis unicinctus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Sephacryl S-100 gel filtration, and Sephadex G-50 gel filtration Urechis unicinctus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Urechis unicinctus ?
-
 ?
fibrinogen + H2O
-
 Urechis unicinctus fibrin + ?
-
 ?
additional information the enzyme was devoid of hemorrhagic effect Urechis unicinctus ?
-
 ?
plasminogen + H2O
-
 Urechis unicinctus plasmin + ?
-
 ?
S-2444 + H2O i.e. pyroGlu-Gly-Arg-4-nitroanilide Urechis unicinctus pyroGlu-Gly-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 20800, SDS-PAGE Urechis unicinctus

Synonyms

Synonyms Comment Organism
fibrinolytic protease
-
 Urechis unicinctus
UFEIII
-
 Urechis unicinctus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
 Urechis unicinctus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme remains stable between 30 and 60°C when incubated for 40 min Urechis unicinctus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 at approximately 55°C Urechis unicinctus

pH Range

pH Minimum pH Maximum Comment Organism
6 10 more than 50% activity between pH 6.0 and 10.0 Urechis unicinctus