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Literature summary for 3.4.24.72 extracted from

  • Majumdar, S.; Sarmah, B.; Gogoi, D.; Banerjee, S.; Ghosh, S.; Banarjee, S.; Chattopadhyay, P.; Mukherjee, A.
    Characterization, mechanism of anticoagulant action, and assessment of therapeutic potential of a fibrinolytic serine protease (Brevithrombolase) purified from Brevibacillus brevis strain FF02B (2014), Biochimie, 103, 50-60.
    View publication on PubMed
Search for more literature for 3.4.24.72

Application

Application Comment Organism
medicine the enzyme has therapeutic potential in peptide-based cardiovascular drug development Brevibacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
4-bromophenacyl bromide 78.5% residual activity at 2 mM Brevibacillus brevis
disodium-EDTA 21.1% residual activity at 4 mM Brevibacillus brevis
dithiothreitol 92.8% residual activity at 4 mM Brevibacillus brevis
iodoacetamide 49.9% residual activity at 4 mM Brevibacillus brevis
N-4-tosyl-phenylalanyl chloromethyl ketone 16.5% residual activity at 0.1 mM Brevibacillus brevis
Nalpha-tosyl-L-lysine chloromethylketone 51.5% residual activity at 0.1 mM Brevibacillus brevis
phenylmethylsulfonyl fluoride 52.9% residual activity at 2 mM Brevibacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 55000, SDS-PAGE Brevibacillus brevis
56043
-
 1 * 56043, MALDI-TOF mass spectrometry Brevibacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrin + H2O Brevibacillus brevis
-
 ?
-
 ?
Fibrin + H2O Brevibacillus brevis FF02B
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
-
-
Brevibacillus brevis FF02B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Hi Prep phenyl column chromatography, and Superdex 75 gel filtration Brevibacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the enzyme shows specific activity of 5.2 units/mg (cell free crude extract) and 64.9 units/mg (after 12.4 fold purification). One unit is defined as microgram of L-tyrosine equivalent liberated per ml per min after 30 min incubation at 37°C Brevibacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bovine plasma fibrinogen + H2O marginal hydrolytic activity Brevibacillus brevis ?
-
 ?
bovine plasma fibrinogen + H2O marginal hydrolytic activity Brevibacillus brevis FF02B ?
-
 ?
bovine serum gamma-globulin + H2O marginal hydrolytic activity Brevibacillus brevis ?
-
 ?
casein + H2O marginal hydrolytic activity Brevibacillus brevis ?
-
 ?
casein + H2O marginal hydrolytic activity Brevibacillus brevis FF02B ?
-
 ?
Fibrin + H2O
-
 Brevibacillus brevis ?
-
 ?
Fibrin + H2O
-
 Brevibacillus brevis FF02B ?
-
 ?
additional information no activity with plasminogen, N-benzoyl-Pro-Phe-Arg-4-nitroanilide, N-benzoyl-Ile-Glu-Gly-Arg-4-nitroanilide, N-(4-tosyl)-Gly-Pro-Arg-4-nitroanilide, and bovine serum albumin Brevibacillus brevis ?
-
 ?
additional information no activity with plasminogen, N-benzoyl-Pro-Phe-Arg-4-nitroanilide, N-benzoyl-Ile-Glu-Gly-Arg-4-nitroanilide, N-(4-tosyl)-Gly-Pro-Arg-4-nitroanilide, and bovine serum albumin Brevibacillus brevis FF02B ?
-
 ?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O marginal hydrolytic activity Brevibacillus brevis Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O marginal hydrolytic activity Brevibacillus brevis FF02B Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Brevibacillus brevis
monomer 1 * 56043, MALDI-TOF mass spectrometry Brevibacillus brevis

Synonyms

Synonyms Comment Organism
brevithrombolase
-
 Brevibacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Brevibacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Brevibacillus brevis