Cloned (Comment) | Organism |
---|---|
co-expression of the active signal-less His-tagged enzyme with chaperone DsbC in Escherichia coli cytoplasm, over 90% of the recombinant enzyme is soluble due to DsbC, while the chaperones FkpA and Skp do not have an effect on fibrolase solubility | Agkistrodon contortrix contortrix |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Agkistrodon contortrix contortrix | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc metalloprotease | Agkistrodon contortrix contortrix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | Agkistrodon contortrix contortrix | degradation of fibrin clots | ? | - |
? | |
fibrinogen + H2O | Agkistrodon contortrix contortrix | bovine substrate | fibrin + propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agkistrodon contortrix contortrix | P28891 | southern copperhead snake | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity and hydrophobic interaction chromatography to 95.6% purity | Agkistrodon contortrix contortrix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Agkistrodon contortrix contortrix | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrin + H2O | degradation of fibrin clots | Agkistrodon contortrix contortrix | ? | - |
? | |
Fibrin + H2O | fibrinolytic activity | Agkistrodon contortrix contortrix | ? | - |
? | |
fibrinogen + H2O | bovine substrate | Agkistrodon contortrix contortrix | fibrin + propeptide | - |
? | |
fibrinogen + H2O | bovine substrate, fibrinogenolytic activity | Agkistrodon contortrix contortrix | fibrin + propeptide | - |
? | |
additional information | the enzyme is a nonhemorrhagic zinc metalloprotease | Agkistrodon contortrix contortrix | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
snake venom fibrinolytic enzyme | - |
Agkistrodon contortrix contortrix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Agkistrodon contortrix contortrix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Agkistrodon contortrix contortrix |