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Literature summary for 3.4.24.7 extracted from

  • Zhang, Y.; Gray, R.D.
    Characterization of folded, intermediate, and unfolded states of recombinant human interstitial collagenase (1996), J. Biol. Chem., 271, 8015-8021.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme forms insoluble inclusion bodies when over-expressed in Escherichia coli Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the propeptide, which comprises 20% of the mass of the proenzyme, is not required for folding to a functional enzyme Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
optimal folding occurs when the denatured protein is diluted at 4°C in 2 M guanidine HCl, 20% glycerol, 2.5 mM reduced and oxidized glutathione, 5 mM CaCl2, followed by buffer exchange to remove denaturant and thiols Homo sapiens