Cloned (Comment) | Organism |
---|---|
the enzyme forms insoluble inclusion bodies when over-expressed in Escherichia coli | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the propeptide, which comprises 20% of the mass of the proenzyme, is not required for folding to a functional enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Renatured (Comment) | Organism |
---|---|
optimal folding occurs when the denatured protein is diluted at 4°C in 2 M guanidine HCl, 20% glycerol, 2.5 mM reduced and oxidized glutathione, 5 mM CaCl2, followed by buffer exchange to remove denaturant and thiols | Homo sapiens |