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Literature summary for 3.4.24.7 extracted from

  • Lin, T.Y.; Kuo, D.W.
    Inactivation of human fibroblast collagenase by chloroacetyl N-hydroxypeptide derivatives (1991), J. Enzyme Inhib., 5, 33-40.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Trypsin procollagenase activated to collagenase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ClCH2CO-(N-OH)Leu-Ala-Gly-NH2 2-5 mM, 25°C, pH 7.4, Tris buffer, strong irreversible inhibition, inhibition increases with higher temperatures and inhibitor concentration Homo sapiens
ClCH2CO-(N-OH)Phe-Ala-Ala-NH2
-
Homo sapiens
phthaloyl-Gly(P)-Ile-Trp-NHBzl 50 µM, 25°C, pH 7.4, Tris buffer, reversible inhibition, protects the enzyme partially from inactivation by ClCH2CO-(N-OH)Leu-Ala-Gly-NH2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
calculated Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification procollagenase activated by treatment with trypsin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast gingival Homo sapiens
-

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
ClCH2CO-(N-OH)Leu-Ala-Gly-NH2 25°C, pH 7.4, Tris buffer Homo sapiens
2.5
-
ClCH2CO-(N-OH)Phe-Ala-Ala-NH2 25°C, pH 7.4, Tris buffer Homo sapiens