Literature summary for 3.4.24.7 extracted from

Chung, L.; Shimokawa, K.I.; Dinakarpandian, D.; Grams, F.; Fields, G.B.; Nagase, H.
Identification of the 183RWTNNFREY191 region as a critical segment of matrix metalloproteinase 1 for the expression of collagenolytic activity (2000), J. Biol. Chem., 275, 29610-29617.

Search for more literature for 3.4.24.7

Cloned(Commentary)

Cloned (Comment)	Organism
MMP-3/MMP-1 chimeras and variants are overexpressed in Escherichia coli, folded from inclusion bodies and isolated as zymogens	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	introduction of various lengths of MMP-1 segments into MMP-3, i.e. stromelysin 1, starting from the C-terminal end. MMP-3/MMP-1 chimeras and variants are overexpressed in Escherichia coli, folded from inclusion bodies and isolated as zymogens. The nine residues 183RWTNNFREY191 located between the fifth beta-strand and the second alpha-helix in the catalytic domain of MMP-1 are critical for the expression of collagenolytic activity	Homo sapiens
R183Q/W184W/T185T/N186K/N187D/F188T/R189T/E190G/Y191T	mutation reduces collagenolytic activity about 10fold	Homo sapiens
Y191T	mutation reduces collagenolytic and gelatinolytic activity about 5fold	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(7-methoxycoumarin-4-yl)-acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(dinitrophenyl)-NH2 + H2O	-	Homo sapiens	(7-methoxycoumarin-4-yl)-acetyl-Arg-Pro-Lys-Pro-Val-Glu + Nva-Trp-Arg-Lys(dinitrophenyl)-NH2	-	?
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O	-	Homo sapiens	(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly + Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2	-	?
Collagen + H2O	183RWTNNFREY191, together with the C-terminal hemopexin domain, is essential for collagenolytic activity but additional structural elements in the catalytic domain are also required. These elements probably act in a concerted manner to cleave the collagen triple helix	Homo sapiens	?	-	?
Gelatin + H2O	-	Homo sapiens	?	-	?

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Release 2023.1 (January 2023)