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Literature summary for 3.4.24.7 extracted from

  • Lauer-Fields, J.L.; Fields, G.B.
    Triple-helical peptide analysis of collagenolytic protease activity (2002), Biol. Chem., 383, 1095-1105.
    View publication on PubMed
Search for more literature for 3.4.24.7

Protein Variants

Protein Variants Comment Organism
DELTA243-450 the KM-value for the alpa1(I)772-786 triple-helical peptide is 3.3fold higher than that of the wild-type enzyme, the turnover number for this substrate is 2.5fold higher Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 [alpha1(I)]2alpha2(I)772-784 triple-helical peptide mutant enzyme E200A Homo sapiens
0.0208
-
 [alpha1(II)769-783] fluorogenic triple-helical peptide-4 wild-type enzyme Homo sapiens
0.0612
-
 [alpha1(II)769-783] fluorogenic triple-helical peptide-3 wild-type enzyme Homo sapiens
0.063
-
 alpha1(I)772-786 triple-helical peptide wild-type enzyme Homo sapiens
0.171
-
 [alpha1(II)769-783] single-stranded peptide-3 wild-type enzyme Homo sapiens
0.2076
-
 alpha1(I)772-786 triple-helical peptide mutant enzyme DELTA243-450 Homo sapiens
0.63
-
 Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln-Arg-Gly-Val-Val-Gly-Leu-Hyp-NH2 wild-type enzyme Homo sapiens
3.3
-
 Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1(I)772-786 triple-helical peptide + H2O
-
 Homo sapiens ?
-
 ?
Collagen + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln + H2O
-
 Homo sapiens Gly-Pro-Gln-Gly + Ile-Ala-Gly-Gln-Gln
-
 ?
Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln-Arg-Gly-Val-Val-Gly-Leu-Hyp-NH2 + H2O
-
 Homo sapiens Gly-Pro-Gln-Gly + Ile-Ala-Gly-Gln-Gln-Arg-Gly-Val-Val-Gly-Leu-Hyp-NH2
-
 ?
[alpha1(I)]2alpha2(I)772-784 triple-helical peptide + H2O
-
 Homo sapiens ?
-
 ?
[alpha1(II)769-783] fluorogenic triple-helical peptide-3 + H2O
-
 Homo sapiens ?
-
 ?
[alpha1(II)769-783] fluorogenic triple-helical peptide-4 + H2O
-
 Homo sapiens ?
-
 ?
[alpha1(II)769-783] single-stranded peptide-3 + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 [alpha1(II)769-783] fluorogenic triple-helical peptide-4 wild-type enzyme Homo sapiens
0.08
-
 [alpha1(II)769-783] fluorogenic triple-helical peptide-3 wild-type enzyme Homo sapiens
0.11
-
 alpha1(I)772-786 triple-helical peptide wild-type enzyme Homo sapiens
0.2
-
 Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln wild-type enzyme Homo sapiens
0.25
-
 [alpha1(II)769-783] single-stranded peptide-3 wild-type enzyme Homo sapiens
0.28
-
 alpha1(I)772-786 triple-helical peptide mutant enzyme DELTA243-450 Homo sapiens
0.51
-
 Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Gln-Arg-Gly-Val-Val-Gly-Leu-Hyp-NH2 wild-type enzyme Homo sapiens