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Literature summary for 3.4.24.69 extracted from
- Endopeptidase activities of botulinum neurotoxin type B complex, holotoxin, and light chain (2010), Appl. Environ. Microbiol., 76, 6658-6663.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Clostridium botulinum |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the endopeptidase activities of the three forms (complex, purified BoNT/B holotoxin, and separated light chain) are compared under the same conditions. Results show that enzyme activities of the three forms differ significantly and are dependent on nicking and disulfide reduction conditions. Light chain form has the highest level of activity, and the complex has the lowest. The activity is enhanced by nicking of BoNT/B holotoxin and is enhanced even more by dithiothreitol reduction after nicking | Clostridium botulinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium botulinum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| VAMPTide + H2O | - |
Clostridium botulinum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BoNT/B | - |
Clostridium botulinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clostridium botulinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Clostridium botulinum |
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Release 2023.1 (January 2023)
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