Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | near-complete conversion of the enzyme SC to a disulfide-linked DC in the presence of DTT | Clostridium botulinum |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged BoNT AE and EA chimeras in Escherichia coli strain BL21 | Clostridium botulinum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of BoNT AE and EA chimeras from serotypes BoNT/A and BoNT/E in DC and SC forms, overview, catalytic and physiological properties of the chimeric forms, overview | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Clostridium botulinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
146000 | - |
recombinant His-tagged BoNT AE and EA chimeras, gel filtration | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium botulinum | the seven serotypes A-G potently block neurotransmission by binding to presynaptic receptors, undergoing endocytosis, transferring to the cytosol, and inactivating proteins essential for vesicle fusion, overview | ? | - |
? | |
SNAP-25 + H2O | Clostridium botulinum | serotypes BoNT/A and BoNT/E cleave SNAP-25 at distinct sites, BoNT/E blocks neurotransmission faster and more potently | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | Q00496 | seven BoNT serotypes A-G | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged BoNT AE and EA chimeras from Escherichia coli strain BL21 by immobilized metal affinity and adsorption chromatography, followed by gel filtration, to homogeneity, near-complete conversion of the SC to a disulfide-linked DC as revealed by the appearance of the HC and LC upon SDS-PAGE in the presence of DTT | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the seven serotypes A-G potently block neurotransmission by binding to presynaptic receptors, undergoing endocytosis, transferring to the cytosol, and inactivating proteins essential for vesicle fusion, overview | Clostridium botulinum | ? | - |
? | |
SNAP-25 + H2O | serotypes BoNT/A and BoNT/E cleave SNAP-25 at distinct sites, BoNT/E blocks neurotransmission faster and more potently | Clostridium botulinum | ? | - |
? | |
SNAP-25 + H2O | substrate is a recombinant GFP-SNAP-25-(134206)-His6 fusion protein | Clostridium botulinum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 146000, recombinant His-tagged BoNT AE and EA chimeras, SDS-PAGE | Clostridium botulinum |
More | near-complete conversion of the SC to a disulfide-linked DCas revealed by the appearance of the HC and LC upon SDS-PAGE in the presence of DTT | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
BoNT | - |
Clostridium botulinum |
botulinum toxin | - |
Clostridium botulinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Clostridium botulinum |