Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.68 extracted from

  • Rummel, A.; Bade, S.; Alves, J.; Bigalke, H.; Binz, T.
    Two carbohydrate binding sites in the H(CC)-domain of tetanus neurotoxin are required for toxicity (2003), J. Mol. Biol., 326, 835-847.
    View publication on PubMed
Search for more literature for 3.4.24.68

Application

Application Comment Organism
medicine causative agent of the disease tetanus Clostridium tetani

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Clostridium tetani

Protein Variants

Protein Variants Comment Organism
D1222L lactose-binding site mutant, mutation generated by PCR Clostridium tetani
G1215F sialic acid binding site mutant, mutation generated by PCR Clostridium tetani
G1300F lactose-binding site mutant, mutation generated by PCR Clostridium tetani
H1271A lactose-binding site mutant, mutation generated by PCR Clostridium tetani
H1271W lactose-binding site mutant, mutation generated by PCR Clostridium tetani
H1293A lactose-binding site mutant, mutation generated by PCR Clostridium tetani
N1219I lactose-binding site mutant, mutation generated by PCR Clostridium tetani
N1220I lactose-binding site mutant, mutation generated by PCR Clostridium tetani
R1226F sialic acid binding site mutant, mutation generated by PCR Clostridium tetani
R1226L sialic acid binding site mutant, mutation generated by PCR Clostridium tetani
S1287A lactose-binding site mutant, mutation generated by PCR Clostridium tetani
W1289G lactose-binding site mutant, mutation generated by PCR Clostridium tetani
W1289L lactose-binding site mutant, mutation generated by PCR Clostridium tetani
Y1290A lactose-binding site mutant, mutation generated by PCR Clostridium tetani
Y1290F lactose-binding site mutant, mutation generated by PCR Clostridium tetani

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent endopeptidase Clostridium tetani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 single-chain protein, cleaved by proteases into a 100 kDa heavy chain HC and a 50 kDa light chain LC, SELDI-TOF MS Clostridium tetani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium tetani most powerful known natural toxin, 2 carbohdrate binding sites in the Hcc-domain of tetanus neurotoxin are required for toxicity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium tetani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutated recombinant Hc-fragments Clostridium tetani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information most powerful known natural toxin, 2 carbohdrate binding sites in the Hcc-domain of tetanus neurotoxin are required for toxicity Clostridium tetani ?
-
 ?
rat synaptobrevin 2 + H2O catalytic activity of all mutants Clostridium tetani ?
-
 ?

Synonyms

Synonyms Comment Organism
TeNT
-
 Clostridium tetani
Tetanus neurotoxin
-
 Clostridium tetani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 Tm-value 58.4°C Clostridium tetani