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Literature summary for 3.4.24.65 extracted from

  • Lamort, A.S.; Gravier, R.; Laffitte, A.; Juliano, L.; Zani, M.L.; Moreau, T.
    New insights into the substrate specificity of macrophage elastase MMP-12 (2016), Biol. Chem., 397, 469-484 .
    View publication on PubMed
Search for more literature for 3.4.24.65

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His-tagged catalytic domain of MMP-12 in Escherichia coli strain BL21(DE3) Mus musculus
recombinant expression of C-terminally His-tagged catalytic domain of MMP-12 in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GM6001
-
 Homo sapiens
GM6001
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0068
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0075
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0088
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0091
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0093
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0103
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0123
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0138
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0144
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0146
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0156
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0197
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.0202
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.0249
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.029
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Mus musculus
-
-
extracellular the enzyme is secreted Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Elastin + H2O Mus musculus
-
 ?
-
 ?
Elastin + H2O Homo sapiens
-
 ?
-
 ?
additional information Mus musculus the catalytic domain of MMP-12 is unique among MMPs in that it is very highly active on numerous substrates including elastin ?
-
 ?
additional information Homo sapiens the catalytic domain of MMP-12 is unique among MMPs in that it is very highly active on numerous substrates including elastin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P39900
-
-
Mus musculus P34960
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged catalytic domain of MMP-12 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Mus musculus
recombinant C-terminally His-tagged catalytic domain of MMP-12 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
bronchial epithelial cell
-
 Mus musculus
-
bronchial epithelial cell
-
 Homo sapiens
-
commercial preparation
-
 Homo sapiens
-
macrophage alveolar Mus musculus
-
macrophage alveolar Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-ASVATE + LRAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-ASVATE + LRAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus ?
-
 ?
2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens ?
-
 ?
2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is E-/-PLA Mus musculus 2-aminobenzoyl-EPLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is E-/-PLA Homo sapiens 2-aminobenzoyl-EPLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage sites are LEAI-/-P-/-MSIP Mus musculus 2-aminobenzoyl-GAMF + LEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage sites are LEAI-/-P-/-MSIP Homo sapiens 2-aminobenzoyl-GAMF + LEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-GPLE + LEEAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-GPLE + LEEAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-GPLG + LERAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-GPLG + LERAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-GPLG + LREAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-GPLG + LREAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is PL-/-A Mus musculus 2-aminobenzoyl-PLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is PL-/-A Homo sapiens 2-aminobenzoyl-PLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-PLG + LEEA-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-PLG + LEEA-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is LW-/-RSQ Mus musculus 2-aminobenzoyl-RGLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is LW-/-RSQ Homo sapiens 2-aminobenzoyl-RGLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RNALA + VERTAS-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RNALA + VERTAS-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLA + LEESQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLA + LEESQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-A Mus musculus 2-aminobenzoyl-RPLA + LRRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-A Homo sapiens 2-aminobenzoyl-RPLA + LRRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLA + LWEEQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLA + LWEEQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLA + LWESQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLA + LWESQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-A Mus musculus 2-aminobenzoyl-RPLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-A Homo sapiens 2-aminobenzoyl-RPLA + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-E Mus musculus 2-aminobenzoyl-RPLE + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-E Homo sapiens 2-aminobenzoyl-RPLE + LWRSQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLG + LEEA-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLG + LEEA-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLG + LGAAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLG + LGAAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Mus musculus 2-aminobenzoyl-RPLG + LGGAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O
-
 Homo sapiens 2-aminobenzoyl-RPLG + LGGAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-G Mus musculus 2-aminobenzoyl-RPLG + LWGAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide + H2O minor cleavage site is RPL-/-G Homo sapiens 2-aminobenzoyl-RPLG + LWGAQ-2-(2,4-dinitrophenyl)aminoethylamide
-
 ?
Elastin + H2O
-
 Mus musculus ?
-
 ?
Elastin + H2O
-
 Homo sapiens ?
-
 ?
additional information the catalytic domain of MMP-12 is unique among MMPs in that it is very highly active on numerous substrates including elastin Mus musculus ?
-
 ?
additional information the catalytic domain of MMP-12 is unique among MMPs in that it is very highly active on numerous substrates including elastin Homo sapiens ?
-
 ?
additional information substrate specificity analysis using several peptide substrates, peptide design and evaluation, docking study, detailed overview Mus musculus ?
-
 ?
additional information substrate specificity analysis using several peptide substrates, peptide design and evaluation, docking study, detailed overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-12
-
 Mus musculus
MMP-12
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.033
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.11
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.17
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.19
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.26
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.29
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
0.55
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
0.85
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
1.3
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
1.4
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
1.5
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
2.4
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
2.8
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
3.8
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
5.5
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function macrophage elastase, or MMP-12, is mainly produced by alveolar macrophages and is believed to play a major role in the development of chronic obstructive pulmonary disease (COPD). MMP-12 is predominantly produced by activated macrophages at sites of inflammation. MMP-12 seems to also have a function in regulating antiviral immunity. The protease may enter the nucleus of a virus-infected cell where it serves as a transcription factor driving the transcription of the IkappaBalpha gene. This results in the secretion of the antiviral molecule interferon-alpha. The second, distinct role of MMP-12 in antiviral immunity is to proteolytically clear interferon-alpha from the blood, so allowing the virus-induced inflammation to be resolved Mus musculus
physiological function macrophage elastase, or MMP-12, is mainly produced by alveolar macrophages and is believed to play a major role in the development of chronic obstructive pulmonary disease (COPD). MMP-12 is predominantly produced by activated macrophages at sites of inflammation. MMP-12 seems to also have a function in regulating antiviral immunity. The protease may enter the nucleus of a virus-infected cell where it serves as a transcription factor driving the transcription of the IkappaBalpha gene. This results in the secretion of the antiviral molecule interferon-alpha. The second, distinct role of MMP-12 in antiviral immunity is to proteolytically clear interferon-alpha from the blood, so allowing the virus-induced inflammation to be resolved Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide below, full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
2
-
 2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
2
-
 2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
2
-
 2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
2
-
 2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide below, recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
2
-
 2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2.3
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
2.7
-
 2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
3.2
-
 2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
3.6
-
 2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
3.9
-
 2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
4.7
-
 2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
5.9
-
 2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
6
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
6.2
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
6.2
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
7.3
-
 2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
7.4
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
7.4
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
8.4
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
8.5
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
9.3
-
 2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
10
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
10
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
11
-
 2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
13
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
17
-
 2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
23
-
 2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
24
-
 2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
27
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
32
-
 2-aminobenzoyl-RGLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
37
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
38
-
 2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Mus musculus
50
-
 2-aminobenzoyl-EAAPSSVIAATE-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
55
-
 2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
59
-
 2-aminobenzoyl-RPLGLGGAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
61
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
64
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
69
-
 2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
71
-
 2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
74
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
74
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
82
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide recombinant catalytic domain of MMP-12, pH 7.5, 30°C Homo sapiens
110
-
 2-aminobenzoyl-PLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
110
-
 2-aminobenzoyl-GAMFLEAIPMSIP-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
140
-
 2-aminobenzoyl-RNALAVERTAS-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
145
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Mus musculus
170
-
 2-aminobenzoyl-ASVATELRAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
190
-
 2-aminobenzoyl-PLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
280
-
 2-aminobenzoyl-GPLELEEAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
310
-
 2-aminobenzoyl-RPLGLGAAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
390
-
 2-aminobenzoyl-GPLGLERAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
420
-
 2-aminobenzoyl-RPLALWEEQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
560
-
 2-aminobenzoyl-RPLALWESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
620
-
 2-aminobenzoyl-EPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
660
-
 2-aminobenzoyl-GPLGLREAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
700
-
 2-aminobenzoyl-RPLGLWGAQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
720
-
 2-aminobenzoyl-RPLGLEEA-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
730
-
 2-aminobenzoyl-RPLALRRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
760
-
 2-aminobenzoyl-RPLELWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
920
-
 2-aminobenzoyl-RPLALWRSQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens
1600
-
 2-aminobenzoyl-RPLALEESQ-2-(2,4-dinitrophenyl)aminoethylamide full-length enzyme MMP-12, pH 7.5, 30°C Homo sapiens