Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for drug development | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of the MMP-12 catalytic domain, residues Phe100-Gly263, in Escherichia coli strain BL21(DE3) inclusion bodies | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme plays an important role in inflammatory processes and is involved in a number of physiological or pathological situations, such as conversion of plasminogen into angiostatin, allergic airway inflammation, vascular remodeling or alteration, as well as emphysema | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
refolded recombinant MMP-12 catalytic domain from Escherichia coli strain BL21(DE3) inclusion bodies by anion exchange chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant MMP-12 catalytic domain from Escherichia coli strain BL21(DE3) inclusion bodies with refolding buffer, which contains 6 M urea, 20 mM Tris-HCl, 5 mM CaCl2, and 100 mM NaCl, pH 7.5, followed by a two-step dialysis against refolding buffer containing 0.1 mM ZnCl2, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O | - |
Homo sapiens | Mca-Pro-Leu-Gly + Leu-Dpa-Ala-Arg-NH2 | - |
? | |
additional information | the enzyme plays an important role in inflammatory processes and is involved in a number of physiological or pathological situations, such as conversion of plasminogen into angiostatin, allergic airway inflammation, vascular remodeling or alteration, as well as emphysema | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | NMR and mass spectrometry structure determination and anaylsis of the MMP-12 catalytic domain using the refolded recombinantly expressed protein, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
human macrophage elastase | - |
Homo sapiens |
MMP-12 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |