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Literature summary for 3.4.24.65 extracted from

  • Zheng, X.; Ou, L.; Tong, X.; Zhu, J.; Wu, H.
    Over-expression and refolding of isotopically labeled recombinant catalytic domain of human macrophage elastase (MMP-12) for NMR studies (2007), Protein Expr. Purif., 56, 160-166.
    View publication on PubMed

Application

Application Comment Organism
drug development the enzyme is a target for drug development Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the MMP-12 catalytic domain, residues Phe100-Gly263, in Escherichia coli strain BL21(DE3) inclusion bodies Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme plays an important role in inflammatory processes and is involved in a number of physiological or pathological situations, such as conversion of plasminogen into angiostatin, allergic airway inflammation, vascular remodeling or alteration, as well as emphysema ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
refolded recombinant MMP-12 catalytic domain from Escherichia coli strain BL21(DE3) inclusion bodies by anion exchange chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
recombinant MMP-12 catalytic domain from Escherichia coli strain BL21(DE3) inclusion bodies with refolding buffer, which contains 6 M urea, 20 mM Tris-HCl, 5 mM CaCl2, and 100 mM NaCl, pH 7.5, followed by a two-step dialysis against refolding buffer containing 0.1 mM ZnCl2, overview Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O
-
Homo sapiens Mca-Pro-Leu-Gly + Leu-Dpa-Ala-Arg-NH2
-
?
additional information the enzyme plays an important role in inflammatory processes and is involved in a number of physiological or pathological situations, such as conversion of plasminogen into angiostatin, allergic airway inflammation, vascular remodeling or alteration, as well as emphysema Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More NMR and mass spectrometry structure determination and anaylsis of the MMP-12 catalytic domain using the refolded recombinantly expressed protein, overview Homo sapiens

Synonyms

Synonyms Comment Organism
human macrophage elastase
-
Homo sapiens
MMP-12
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens