Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.62 extracted from

  • Clamagirand, C.; Creminon, C.; Fahy, C.; Boussetta, H.; Cohen, P.
    Partial purification and functional properties of an endoprotease from bovine neurosecretory granules cleaving proocytocin/neurophysin peptides at the basic amino acid doublet (1987), Biochemistry, 26, 6018-6023.
    View publication on PubMed
Search for more literature for 3.4.24.62

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak, 1 mM, peptide I as substrate Bos taurus
Cd2+ weak, 0.1 mM, peptide I as substrate Bos taurus
EDTA peptide I as substrate; strong, 2.5 mM Bos taurus
EGTA peptide I as substrate; strong, 2.5 mM Bos taurus
additional information Mn2+, Mg2+, Cu2+; no inhibition by PMSF, pepstatin Bos taurus
p-chloromercuriphenylsulfonic acid 2.5 mM; peptide I as substrate Bos taurus
PCMB 2.5 mM; peptide I as substrate Bos taurus
Zn2+ 1 mM, peptide I as substrate Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloendopeptidase Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000 58000 bovine, gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from purified vesicles Bos taurus
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
hypophysis pituitary neurosecretory granule Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information No substrates are Nle11 or Nle12 Bos taurus ?
-
 ?
additional information No substrates are D-Lys11 Bos taurus ?
-
 ?
additional information No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12 Bos taurus ?
-
 ?
Pro-oxytocin/neurophysin(1-20) + H2O synthetic eicosapeptide reproducing the entire sequence of amino-terminal domain of bovine proOc/Np precursor Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
Pro-oxytocin/neurophysin(1-20) + H2O strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13 Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
Pro-oxytocin/neurophysin(1-20) + H2O i.e. model substrate peptide I Bos taurus Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
 ?
[Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O i.e. peptide XI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20) Bos taurus ?
-
 ?
[Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20) Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.9 and 7.2 (2 isoforms) Bos taurus
7
-
 around, peptide I as substrate Bos taurus