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Literature summary for 3.4.24.61 extracted from

  • Csuhai, E.; Juliano, M.A.; Juliano, L.; Hersh, L.B.
    Kinetic analysis of spermine binding to NRD convertase (1999), Arch. Biochem. Biophys., 362, 291-300.
    View publication on PubMed
Search for more literature for 3.4.24.61

Inhibitors

Inhibitors Comment Organism Structure
2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine substrate inhibitionl Rattus norvegicus
spermine
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00023
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 6.5, 34°C Rattus norvegicus
0.00031
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 8.0, 34°C Rattus norvegicus
0.0015
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 6.0, 34°C Rattus norvegicus
0.00192
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 8.0, 34°C, 25 mM KCl Rattus norvegicus
0.002
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 6.5, 34°C, 50 mM KCl Rattus norvegicus
0.0158
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 6.5, 34°C, 500 mM KCl Rattus norvegicus
0.03
-
 2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine pH 8.2, 34°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
secretory granule
-
 Rattus norvegicus 30141
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ Zn2+-metallopeptidase Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-GGFLRRVGQ-N[2,4-dinitrophenyl]-ethylenediamine + H2O
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
N-Arginine dibasic convertase
-
 Rattus norvegicus
NRD convertase
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 half-life of 5.5 min at pH 6.5 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 9
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.8 enzyme is irreversibly inactivated below pH 6.0 and above pH 9.8 Rattus norvegicus