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Literature summary for 3.4.24.60 extracted from

  • Carvalho, K.M.; Joudiou, C.; Boussetta, H.; Leseney, A.M.; Cohen, P.
    A peptide-hormone-inactivating endopeptidase in Xenopus laevis skin secretion (1992), Proc. Natl. Acad. Sci. USA, 89, 84-88.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.60

General Stability

General Stability Organism
Resistant to 1.5 M NaCl and 30% v/v methanol Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline metalloendopeptidase character Xenopus laevis
Acetorphan less efficient than phosphoramidon Xenopus laevis
benzamidine not Xenopus laevis
EDTA metalloendopeptidase character Xenopus laevis
EGTA metalloendopeptidase character Xenopus laevis
Kelatorphan less efficient than phosphoramidon Xenopus laevis
additional information pepstatin, guanidylethylmercaptosuccinic acid (i.e. GEMSA), captopril, aprotinin, soybean trypsin inhibitor (i.e. STI), PMSF, IAA; retrothiorphan Xenopus laevis
N-ethylmaleimide not Xenopus laevis
p-chloromercuriphenylsulfonic acid not Xenopus laevis
Pentapeptides reproducing or mimicking the COOH-terminal sequence of ANF 24-28 ANF as substrate Xenopus laevis
phosphoramidon only at high concentrations, above 0.001 mM Xenopus laevis
thiorphan i.e. 3-mercapto-2-benzyl-propanoylglycine, only at high concentrations, above 0.001 mM Xenopus laevis
tosyl-Arg methyl ester not Xenopus laevis
[Leu5]enkephalin i.e. Tyr-Gly-Gly-Phe-Leu, ANF as substrate Xenopus laevis
[Leu5]enkephalinamide ANF as substrate Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 Somatostatin-14
-
 Xenopus laevis
0.02
-
 Substance P
-
 Xenopus laevis
0.021
-
 Neuromedin B
-
 Xenopus laevis
0.022
-
 Neuromedin C
-
 Xenopus laevis
0.024
-
 Litorin
-
 Xenopus laevis
0.041
-
 bradykinin
-
 Xenopus laevis
0.05
-
 (5-28)ANF
-
 Xenopus laevis
0.063
-
 angiotensin II
-
 Xenopus laevis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloendopeptidase Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, Xenopus laevis, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Xenopus laevis might be involved in inactivation of a great number of peptide hormones ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Methanol resistant to 30% v/v methanol Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
sweat
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1826
-
 [D-Arg8]-kermit as substrate Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5-28)ANF + H2O i.e. atrial natriuretic factor, cleavage site: exclusively Ser25-Phe26 Xenopus laevis ANF(5-25) + Phe26-Arg27-Tyr28
-
 ?
angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 Xenopus laevis ?
-
 ?
bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, major cleavage site: Gly4-Phe5, minor site: Pro7-Pro8 Xenopus laevis ?
-
 ?
Litorin + H2O minor sites: Gly7-His8 Xenopus laevis ?
-
 ?
Litorin + H2O major cleavage sites: His8-Phe9 Xenopus laevis ?
-
 ?
additional information substrate specificity Xenopus laevis ?
-
 ?
additional information exhibits preference for cleavage on amino-terminal side of Phe, Leu or Ile in Xaa-Leu, Xaa-Phe or Xaa-Ile dipeptide Xenopus laevis ?
-
 ?
additional information no cleavage of analogous bonds in [Leu5]-enkephalin, oxytocin, vasopressin Xenopus laevis ?
-
 ?
additional information cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile Xenopus laevis ?
-
 ?
additional information [Leu5]enkephalin amide, minigastrin I Xenopus laevis ?
-
 ?
additional information might be involved in inactivation of a great number of peptide hormones Xenopus laevis ?
-
 ?
Neuromedin B + H2O i.e. Gly-Asn-Leu-Trp-Ala-Thr-Gly-His-Phe-Met-NH2, major cleavage site: His8-Phe9, minor site: Gly7-His8 Xenopus laevis ?
-
 ?
Neuromedin C + H2O Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2, major cleavage site: His8-Leu9, minor site: Gly7-His8 Xenopus laevis ?
-
 ?
Somatostatin-14 + H2O i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product Xenopus laevis ?
-
 ?
Substance P + H2O major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 Xenopus laevis ?
-
 ?
[D-Arg8]-kermit + H2O model substrate, derivative of kermit i.e. Asp-Val-Asp-Glu-Arg-Asp-Val-Arg-Gly-Phe-Ala-Ser-Phe-Leu-NH2, cleavage site: Ser12-Phe13 Xenopus laevis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, Xenopus laevis, SDS-PAGE Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Xenopus laevis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 about half-maximal activity at pH 6 and about 75% of maximal activity at pH 8 Xenopus laevis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 24 h stable Xenopus laevis