Literature summary for 3.4.24.56 extracted from

Song, E.S.; Jang, H.; Guo, H.F.; Juliano, M.A.; Juliano, L.; Morris, A.J.; Galperin, E.; Rodgers, D.W.; Hersh, L.B.
Inositol phosphates and phosphoinositides activate insulin-degrading enzyme, while phosphoinositides also mediate binding to endosomes (2017), Proc. Natl. Acad. Sci. USA, 114, E2826-E2835 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
1-diphosphoinositol pentakisphosphate	activates, maximal 79.7fold activation	Rattus norvegicus
5-diphosphoinositol pentakisphosphate	activates, maximal 94.7fold activation	Rattus norvegicus
myoinositol 1,2,3,4,5,6-hexakisphosphate	i.e. phytic acid, maximal 72fold activation	Rattus norvegicus
myoinositol 1,2-bisphosphate	activates, maximal 3.1fold activation	Rattus norvegicus
myoinositol 1,3,4,5,6-pentakisphosphate	activates, maximal 83.3fold activation	Rattus norvegicus
myoinositol 1,3,4,5-tetrakisphosphate	activates, maximal 58.6fold activation	Rattus norvegicus
myoinositol 1,3,5-trisphosphate	activates, maximal 12.9fold activation	Rattus norvegicus
myoinositol 1,3-bisphosphate	activates, maximal 6.1fold activation	Rattus norvegicus
myoinositol 1,4,5-trisphosphate	activates, maximal 30.6fold activation	Rattus norvegicus
myoinositol 3-phosphate	activates, maximal 6.2fold activation	Rattus norvegicus
myoinositol 4,5-bisphosphate	activates, maximal 13.8fold activation	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
K898A/K899A/S901A	variant with mutations in the polyanion-binding site shows reduced activation by myoinositol 1,4,5-trisphosphate and phytic acid	Rattus norvegicus
Y609F	activation by myoinositol 1,4,5-trisphosphate and phytic acid is decreased in the mutant enzyme	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0111	-	Abz-Gly-Gly-Leu-Arg-Lys-His-Gly-Gln-EDDnp	37°C, pH 7.4, without activator	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endosome	binding to phosphatdidylinositol phosphates facilitates its localization to endosomes	Rattus norvegicus	5768	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P35559	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-Gly-Gly-Leu-Arg-Lys-His-Gly-Gln-EDDnp + H2O	-	Rattus norvegicus	?	-	?
amyloid beta-peptide(1-40) + H2O	-	Rattus norvegicus	?	-	?
angiotensin + H2O	-	Rattus norvegicus	?	-	?
beta-endorphin + H2O	-	Rattus norvegicus	?	-	?
bradykinin + H2O	-	Rattus norvegicus	?	-	?
dynorphin B9 + H2O	-	Rattus norvegicus	?	-	?
Glucagon + H2O	-	Rattus norvegicus	?	-	?
insulin + H2O	-	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
IDE	-	Rattus norvegicus
Insulin-degrading enzyme	-	Rattus norvegicus

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Release 2023.1 (January 2023)