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Literature summary for 3.4.24.56 extracted from
- Anion activation site of insulin-degrading enzyme (2012), J. Biol. Chem., 287, 48-57.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K898A/K899A/S901A | mutant shows greatly decreased activation by the polyphosphate anions ATP and PPP, mutant is also deficient in activation by small peptides and has reduced intracellular function relative to unmodified IDE. Km and kcat (Abz-GGFLRKHGQ-EDDnp) higher than wild-type | Rattus norvegicus |
| R429S | mutant shows greatly decreased activation by the polyphosphate anions ATP and PPP. kcat (Abz-GGFLRKHGQ-EDDnp) comparable to wild-type and Km (Abz-GGFLRKHGQ-EDDnp) higher than wild-type | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.6 | - |
Abz-GGFLRKHGQ-EDDnp | wild-type, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
| 17.3 | - |
Abz-GGFLRKHGQ-EDDnp | mutant K898A/K899A/S901A, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
| 21.3 | - |
Abz-GGFLRKHGQ-EDDnp | mutant R429S, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activates IDE at high concentration | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Abz-GGFLRKHGQ-EDDnp + H2O | - |
Rattus norvegicus | Abz-GGFLR + KHGQ-EDDnp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDE | - |
Rattus norvegicus |
| Insulysin | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
Abz-GGFLRKHGQ-EDDnp | mutant R429S, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
| 0.086 | - |
Abz-GGFLRKHGQ-EDDnp | wild-type, pH 7.4, temperature not specified in the publication | Rattus norvegicus | |
| 0.258 | - |
Abz-GGFLRKHGQ-EDDnp | mutant K898A/K899A/S901A, pH and temperature not specified in the publication | Rattus norvegicus | |
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