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Literature summary for 3.4.24.56 extracted from

  • Zhang, W.J.; Luo, X.; Guo, Z.Y.
    In vitro degradation of insulin-like peptide 3 by insulin-degrading enzyme (2010), Protein J., 29, 93-98.
    View publication on PubMed
Search for more literature for 3.4.24.56

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline a Zn2+ chelator Homo sapiens
insulin-like peptide 3 IDE degrades insulin quickly, and addition of INSL3 significantly decreases insulin degradation, competitive inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Homo sapiens
0.000052
-
 Insulin pH 7.7, 37°C Homo sapiens
0.000055
-
 insulin-like peptide 3 pH 7.7, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on, IDE is a zinc-metalloprotease Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid beta + H2O Homo sapiens
-
 amyloid beta peptide fragments
-
 ?
insulin + H2O Homo sapiens
-
 insulin peptide fragments
-
 ?
insulin-like peptide 3 + H2O Homo sapiens IDE cleaves the peptide bond between R26 and W27 of the B-chain, and releases a pentapeptide, WSTEA, from the C-terminal of the B-chain processed insulin-like peptide 3 + WSTEA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid beta + H2O
-
 Homo sapiens amyloid beta peptide fragments
-
 ?
insulin + H2O
-
 Homo sapiens insulin peptide fragments
-
 ?
insulin-like peptide 3 + H2O IDE cleaves the peptide bond between R26 and W27 of the B-chain, and releases a pentapeptide, WSTEA, from the C-terminal of the B-chain Homo sapiens processed insulin-like peptide 3 + WSTEA
-
 ?
insulin-like peptide 3 + H2O IDE cleaves the peptide bond between R26 and W27 of the B-chain, and releases a pentapeptide, WSTEA, from the C-terminal of the B-chain, cleavage product identification by mass spectrometry, INSL-3 structure, overview Homo sapiens processed insulin-like peptide 3 + WSTEA
-
 ?

Synonyms

Synonyms Comment Organism
IDE
-
 Homo sapiens
Insulin-degrading enzyme
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
 insulin-like peptide 3 pH 7.7, 37°C Homo sapiens
0.05
-
 Insulin pH 7.7, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function responsible for in vivo degradation of insulin, amyloid beta, and other peptide hormones, e.g. of insulin-like peptide 3, INSL3, an insulin superfamily peptide hormone, primarily expressed in the testes and playing a key role in the fetus testes descent and suppression of male germ cell apoptosis Homo sapiens