Cloned (Comment) | Organism |
---|---|
expression of His-tagged IDE in Escherichia coli strain Rosetta (DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant E110Q with bound insulin, hanging drop vapor diffusion method, 0.001 ml of 16-20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, is mixed with 0.001 ml of reservoir solution containing, 10-13% PEG MME 5000, 100 mM HEPES, pH 7.0, 4-14% tacsimate, and 10% dioxane, equilibration over 0.5 ml of reservoir solution, 18°C, 3-5 days, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, molecular replacement | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E110Q | structure comparison to wild-type enzyme structure | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
insulin + H2O | Homo sapiens | - |
insulin peptide fragments | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged IDE from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Degradation of insulin, glucagon and other polypeptides. No action on proteins | molecular basis of catalytic chamber-assisted unfolding and cleavage of human insulin by human insulin-degrading enzyme, IDE utilizes the interaction of its exosite with the N-terminus of the insulin A chain, overview. The unique size, shape, charge distribution, and exosite of the IDE catalytic chamber contribute to its high affinity for insulin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl + H2O | fluorogenic bradykinin-mimetic IDE substrate V | Homo sapiens | ? | - |
? | |
insulin + H2O | - |
Homo sapiens | insulin peptide fragments | - |
? | |
insulin + H2O | IDE forms an enclosed catalytic chamber that completely engulfs and intimately interacts with a partially unfolded insulin molecule. The unique size, shape, charge distribution, and exosite of the IDE catalytic chamber contribute to its high affinity for insulin, IDE-insulin binding structure and interaction analysis, overview | Homo sapiens | insulin peptide fragments | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Homo sapiens |
Insulin-degrading enzyme | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |