Crystallization (Comment) | Organism |
---|---|
in complex with bradykinin, to 1.9 A resolution. Bradykinin binds to the exosite. Residue C819 is located inside the catalytic chamber pointing toward an extended hydrophobic pocket. Specific activity similar to wild-type using substrate 7-methoxycoumarin-4-ylacetyl-NPPGFSAFK-2,4-dinitrophenyl | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C819A | thiol-directed modification of C819 likely causes local structure perturbation to reduce substrate binding and catalysis | Homo sapiens |
E111Q | catalysitcally inactive. Mutant forms ordered crystals of considerable size at a more rapid rate than the wild type | Homo sapiens |
additional information | construction of mutants lacking one or severall cysteine resiudes. A mutant devoid of all 13 cysteine residues is insensitive to the inhibition by S-nitrosoglutathione, hydrogen peroxide, or N-ethylmaleimide | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrogen peroxide | - |
Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens | |
S-nitrosoglutathione | potent inhibition at physiologically relevant concentrations | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
bradykinin | pH 7.3, 37°C | Homo sapiens | |
7.3 | - |
kallidin | pH 7.3, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-ylacetyl-NPPGFSAFK-2,4-dinitrophenyl + H2O | - |
Homo sapiens | ? | - |
? | |
bradykinin + H2O | cleavage at Pro/Phe site | Homo sapiens | ? | - |
? | |
kallidin + H2O | cleavage at Pro/Phe site | Homo sapiens | ? | - |
? |