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Literature summary for 3.4.24.56 extracted from
- The insulysin (insulin degrading enzyme) enigma (2006), Cell. Mol. Life Sci., 63, 2432-2434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Mus musculus |
| a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Homo sapiens |
| a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H18R/A890V | naturally occuring mutations in the insulysin gene in GK rats causing 30% reduced enzyme activity and type 2 diabetes as well as increased amyloid beta peptide levels , the rats exhibit defects in both insulin action and insulin degradation, the increased amyloid beta-peptide levels do not lead to increased steady-state levels of its activity due to compensatory degradative mechanisms in the brain | Rattus norvegicus |
| additional information | overexpression of insulysin in human amyloid precursor protein transgenic mice leads to a decrease in amyloid beta peptide levels | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
| cytosol | primarily | Mus musculus | 5829 | - |
| cytosol | primarily | Homo sapiens | 5829 | - |
| cytosol | primarily | Rattus norvegicus | 5829 | - |
| mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Mus musculus | 5739 | - |
| mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Homo sapiens | 5739 | - |
| mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Rattus norvegicus | 5739 | - |
| peroxisome | - |
Mus musculus | 5777 | - |
| peroxisome | - |
Homo sapiens | 5777 | - |
| peroxisome | - |
Rattus norvegicus | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Mus musculus |  |
| Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Homo sapiens | |
| Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
about | Mus musculus |
| 110000 | - |
about | Homo sapiens |
| 110000 | - |
about | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta-peptide + H2O | Mus musculus | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
| amyloid beta-peptide + H2O | Homo sapiens | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
| amyloid beta-peptide + H2O | Rattus norvegicus | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
| insulin + H2O | Mus musculus | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | ? | - |
? | |
| insulin + H2O | Homo sapiens | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | ? | - |
? | |
| insulin + H2O | Rattus norvegicus | degradation, type 2 diabetic GK rats exhibit defects in both insulin action and insulin degradation mainly due to mutation H18R and A890V in the insulysin protein | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
healthy and type 2 diabetic GK rats | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Rattus norvegicus | - |
| endothelial cell | cerebrovascular, the this cell surface form is the major form of insulysin responsible for degrading amyloid beta-peptides | Homo sapiens | - |
| Hep-G2 cell | - |
Homo sapiens | - |
| hepatocyte | primary | Homo sapiens | - |
| hippocampus | insulysin is reduced in the hippocampus of Alzheimer's disease patients with an apolipoprotein E-4 allele | Homo sapiens | - |
| lymphocyte | - |
Homo sapiens | - |
| additional information | insulysin activity declines with aging in the hippocampus of a mouse model of Alzheimers disease | Mus musculus | - |
| neuron | primary cell culture | Rattus norvegicus | - |
| pancreatic acinar cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta-peptide + H2O | degradation | Mus musculus | ? | - |
? | |
| amyloid beta-peptide + H2O | degradation | Homo sapiens | ? | - |
? | |
| amyloid beta-peptide + H2O | degradation | Rattus norvegicus | ? | - |
? | |
| amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Mus musculus | ? | - |
? | |
| amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Homo sapiens | ? | - |
? | |
| amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Rattus norvegicus | ? | - |
? | |
| insulin + H2O | degradation | Mus musculus | ? | - |
? | |
| insulin + H2O | degradation | Homo sapiens | ? | - |
? | |
| insulin + H2O | degradation | Rattus norvegicus | ? | - |
? | |
| insulin + H2O | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | Mus musculus | ? | - |
? | |
| insulin + H2O | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | Homo sapiens | ? | - |
? | |
| insulin + H2O | degradation, type 2 diabetic GK rats exhibit defects in both insulin action and insulin degradation mainly due to mutation H18R and A890V in the insulysin protein | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 110000, about | Mus musculus |
| monomer | 1 * 110000, about | Homo sapiens |
| monomer | 1 * 110000, about | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDE | - |
Mus musculus |
| IDE | - |
Homo sapiens |
| IDE | - |
Rattus norvegicus |
| insulin degrading enzyme | - |
Mus musculus |
| insulin degrading enzyme | - |
Homo sapiens |
| insulin degrading enzyme | - |
Rattus norvegicus |
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