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Literature summary for 3.4.24.56 extracted from

  • Werlen, R.C.; Offord, R.E.; Rose, K.
    Preparation and characterization of novel substrates of insulin proteinase (EC 3.4.99.45) (1994), Biochem. J., 302, 907-911.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Rattus norvegicus
1,10-phenanthroline
-
Sus scrofa
bacitracin
-
Sus scrofa
Co2+
-
Rattus norvegicus
Co2+
-
Sus scrofa
Cu2+
-
Rattus norvegicus
Cu2+
-
Sus scrofa
EDTA
-
Rattus norvegicus
EDTA
-
Sus scrofa
Fragment of cytochrome c
-
Sus scrofa
Mn2+
-
Rattus norvegicus
Mn2+
-
Sus scrofa
additional information not: bestatine Rattus norvegicus
additional information not: benzamidine; not: bestatine; not: phenylmethanesulfonyl fluoride Sus scrofa
NEM
-
Rattus norvegicus
NEM
-
Sus scrofa
Ni2+
-
Rattus norvegicus
Ni2+
-
Sus scrofa
Tryptic fragment of bovine serum albumin
-
Sus scrofa
Zn2+
-
Rattus norvegicus
Zn2+
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Rattus norvegicus
Ca2+ activates Sus scrofa
Mg2+ activates Rattus norvegicus
Mg2+ activates Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
x * 110000, pig, SDS-PAGE Rattus norvegicus
110000
-
x * 110000, pig, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Rattus norvegicus
-
skeletal muscle
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus
additional information
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fragment of cytochrome c + H2O Ile81-Glu108 Sus scrofa Hydrolyzed fragment of cytochrome c cleavage at Tyr97-Leu98 bond ?
Insulin + H2O
-
Rattus norvegicus Hydrolyzed insulin
-
?
Insulin + H2O
-
Sus scrofa Hydrolyzed insulin
-
?
additional information
-
Rattus norvegicus ?
-
?
additional information preference for small substrates 2000-6000 MW Sus scrofa ?
-
?
Tryptic fragment of bovine serum albumin + H2O Leu503-Lys518 Sus scrofa Hydrolyzed tryptic fragment of bovine serum albumin cleaved at Phe506-His507 ?

Subunits

Subunits Comment Organism
? x * 110000, pig, SDS-PAGE Rattus norvegicus
? x * 110000, pig, SDS-PAGE Sus scrofa