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Literature summary for 3.4.24.55 extracted from

  • Cornista, J.; Ikeuchi, S.; Haruki, M.; Kohara, A.; Takano, K.; Morikawa, M.; Kanaya, S.
    Cleavage of various peptides with pitrilysin from Escherichia coli: kinetic analyses using beta-endorphin and its derivatives (2004), Biosci. Biotechnol. Biochem., 68, 2128-2137.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
beta-endorphin pH 8.0, 37°C Escherichia coli
0.0004
-
Vasoactive intestinal peptide pH 8.0, 37°C Escherichia coli
0.0009
-
beta-amyloid pH 8.0, 37°C Escherichia coli
0.0015
-
Insulin B-chain pH 8.0, 37°C Escherichia coli
0.0022
-
calcitonin pH 8.0, 37°C Escherichia coli
0.031
-
Substance P pH 8.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyloid + H2O 18% of the activity with beta-endorphin, major cleavage site His14-Gln15 Escherichia coli ?
-
?
beta-endorphin + H2O cleavage at Lys19-Asn20 bond. Residues Leu14, Val15 and Leu17 and region 22-26 are responsible for recognition by enzyme Escherichia coli ?
-
?
calcitonin + H2O 3% of the activity with beta-endorphin Escherichia coli ?
-
?
Glucagon + H2O 8% of the activity with beta-endorphin Escherichia coli ?
-
?
Insulin B-chain + H2O 17% of the activity with beta-endorphin, major cleavage site Tyr16-Leu17 Escherichia coli ?
-
?
Substance P + H2O 10% of the activity with beta-endorphin Escherichia coli ?
-
?
Vasoactive intestinal peptide + H2O 7.2% of the activity with beta-endorphin, major cleavage site Arg14-Lys15 Escherichia coli ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
calcitonin pH 8.0, 37°C Escherichia coli
0.63
-
Substance P pH 8.0, 37°C Escherichia coli
0.9
-
Vasoactive intestinal peptide pH 8.0, 37°C Escherichia coli
2.33
-
beta-amyloid pH 8.0, 37°C Escherichia coli
2.66
-
Insulin B-chain pH 8.0, 37°C Escherichia coli
12.5
-
beta-endorphin pH 8.0, 37°C Escherichia coli