KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00036 | - |
beta-endorphin | pH 8.0, 37°C | Escherichia coli | |
0.0004 | - |
Vasoactive intestinal peptide | pH 8.0, 37°C | Escherichia coli | |
0.0009 | - |
beta-amyloid | pH 8.0, 37°C | Escherichia coli | |
0.0015 | - |
Insulin B-chain | pH 8.0, 37°C | Escherichia coli | |
0.0022 | - |
calcitonin | pH 8.0, 37°C | Escherichia coli | |
0.031 | - |
Substance P | pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyloid + H2O | 18% of the activity with beta-endorphin, major cleavage site His14-Gln15 | Escherichia coli | ? | - |
? | |
beta-endorphin + H2O | cleavage at Lys19-Asn20 bond. Residues Leu14, Val15 and Leu17 and region 22-26 are responsible for recognition by enzyme | Escherichia coli | ? | - |
? | |
calcitonin + H2O | 3% of the activity with beta-endorphin | Escherichia coli | ? | - |
? | |
Glucagon + H2O | 8% of the activity with beta-endorphin | Escherichia coli | ? | - |
? | |
Insulin B-chain + H2O | 17% of the activity with beta-endorphin, major cleavage site Tyr16-Leu17 | Escherichia coli | ? | - |
? | |
Substance P + H2O | 10% of the activity with beta-endorphin | Escherichia coli | ? | - |
? | |
Vasoactive intestinal peptide + H2O | 7.2% of the activity with beta-endorphin, major cleavage site Arg14-Lys15 | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
calcitonin | pH 8.0, 37°C | Escherichia coli | |
0.63 | - |
Substance P | pH 8.0, 37°C | Escherichia coli | |
0.9 | - |
Vasoactive intestinal peptide | pH 8.0, 37°C | Escherichia coli | |
2.33 | - |
beta-amyloid | pH 8.0, 37°C | Escherichia coli | |
2.66 | - |
Insulin B-chain | pH 8.0, 37°C | Escherichia coli | |
12.5 | - |
beta-endorphin | pH 8.0, 37°C | Escherichia coli |