Protein Variants | Comment | Organism |
---|---|---|
D79A | no effect on function | Escherichia coli |
E91A | no proteolytic activity | Escherichia coli |
F198A | enzyme remains functional | Escherichia coli |
H193A | enzyme remains functional | Escherichia coli |
K197A | enzyme remains functional | Escherichia coli |
additional information | heterologous expression of enzyme in yeast, enzyme functionally substitutes for yeast Ax11p and Ste23p in pheromone production. Independent heterologous expression of N-terminal domain with amino acids 1-515 or C-terminal domain with amino acids 516-962 does not promote yeast mating. Co-expression of both domains restores yeast mating | Escherichia coli |
N119A | promotion of yeast mating factor in heterologous expression is disabled | Escherichia coli |
N317A/R318A/S319A | enzyme remains functional | Escherichia coli |
R792A | no proteolytic activity | Escherichia coli |
R792A/T793A/E794A/E795A | no proteolytic activity | Escherichia coli |
S117A | enzyme remains functional | Escherichia coli |
S117A/H118A/N119A | promotion of yeast mating factor in heterologous expression is disabled | Escherichia coli |
S196A/K197A/F198A/S199A | promotion of yeast mating factor in heterologous expression is disabled | Escherichia coli |
T127A | enzyme remains functional | Escherichia coli |
T322A/L323A | enzyme remains functional | Escherichia coli |
Y799A | no proteolytic activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |